摘要
目的：将既往有关E-钙黏蛋白（CDH1）基因启动子区甲基化与膀胱癌关系的研究进行Meta分析，评估CDH1基因启动子区甲基化与膀胱癌的具体关联。方法参照Cochrane协作网制定的检索策略在国内外相关数据库进行检索，筛选并纳入文献后，提取文献信息：第一作者、文献发表时间、国家、语种、研究设计、样本量、种族、病理亚型、甲基化检测方法、基因甲基化频率等。采用STATA12.0进行Meta分析，计算优势比（OR）及其95%可信区间（95%CI）。结果本Meta分析共计纳入10项病例对照研究，通过检测其中620例膀胱癌组织和341例正常或癌旁组织的CDH1基因甲基化频率，结果发现膀胱癌组织中的甲基化频率明显高于正常或癌旁组织（OR=3.09，95%CI：1.13～8.50，P=0.029）；亚组分析结果显示，在亚洲人群中膀胱癌组织中的甲基化频率明显高于正常或癌旁组织（OR=3.85，95%CI：1.46-10.14，P=0.006），而在欧美人群中未发现此种差异（OR=2.22，95%CI：0.38-12.91，P=0.375）；采用MSP方法检测时，膀胱癌组织与癌旁及正常组织间CDH1基因启动子区甲基化频率存在明显差异（P〈0.001），采用Q-MSP方法检测时，两组CDH1基因启动子区甲基化频率无明显差异（P=0.818）。结论 CDH1基因启动子区甲基化可能参与了膀胱癌的发生和发展。
Objective To assess the role of E-cadherin （CDH1） promoter methylation in bladder carcinogenesis by meta-analysis. Methods The relevant database were searched by the retrieval strategy of Cochrane network. All included studies were collected following data：the first author’s surname, publication year of article, country, language of publication, design of study, sample size, ethnicity, histological subtypes, methylation detection method and genotype frequencies etc. This meta-analysis was performed using the STATA 12.0 software. The crude odds ratio （OR） with 95%confidence interval （CI） was calculated. Results Ten case-control studies were included in this meta-analysis. The methylation frequency of CDH1 was detected in 620 bladder cancer tissues and 341 normal or cancerous tissues. Results showed that the methylation frequency of CDH1 was significantly higher in bladder cancer tissue than that of normal or cancerous tissue （OR=3.09, 95%CI：1.13-8.50, P=0.029）. Furthermore, the ethnicity-stratified analysis revealed that the methylation frequency of CDH1 was significantly higher in bladder cancer tissue of Asian populations than that of normal or cancerous tissue （OR=3.85, 95%CI：1.46-10.14, P=0.006）, but no such association was found in Caucasian populations（OR=2.22, 95%CI：0.38-12.91, P=0.375）. The subgroup analysis based on the detection methods revealed that there was a statistically significant difference in the methylation frequency of CDH1 between bladder cancer tissue and adjacent tissues and normal tissues under the MSP subgroup （P〈0.001）, while such association was not observed under the Q-MSP subgroup （P=0.818）. Conclusion Pro？moter methylation of CDH1 gene may be involved in the occurrence and development of bladder cancer, which may serve as a biomarker for diagnosis and prognosis of bladder cancer.
出处
《天津医药》
CAS
2015年第1期97-101,共5页
Tianjin Medical Journal
