摘要
目的：本研究旨在体外筛选出藏族药翼首草正丁醇部位提取物（YSC-ZDC）抑制人肝癌Hep3B细胞侵袭转移的有效萃取部位,并对其抗肿瘤作用机制进行初步探讨。方法：取对数生长期Hep3B细胞,调整细胞密度为4×10^4/m L（MTT法）或2.5×105/m L（Western blot,heochst 33258实验）,1×10^5/m L（划痕实验）,5×10^4/m L（Transwell实验）,培养24,48,72 h后分别加入不同质量浓度的药物（50,100,200 mg·L^-1）,分组设3-4个复孔。通过MTT法检测YSC-ZDC对于Hep3B细胞增殖的影响,形态观察该药对Hep3B细胞凋亡的影响。划痕实验和Transwell小室检测YSC-ZDC对Hep3B细胞侵袭、迁移的影响;Western blot检测侵袭转移相关信号通路分子表达的变化。结果：YSC-ZDC 50,100,200 mg·L^-1作用于Hep3B细胞24,48,72 h后可明显抑制其增长。50,100,200 mg·L^-1YSC-ZDC作用于Hep3B细胞24 h后可明显抑制其侵袭和迁移;YSC-ZDC可明显影响上皮-间质转化（EMT）相关蛋白E-钙黏素,N-钙黏素（E-cadherin,N-cadherin）和纤维连接蛋白（FN）的表达（P〈0.05）。结论：YSC-ZDC能显著抑制Hep3B细胞的增殖和侵袭转移,并诱导Hep3B细胞凋亡,其作用机制可能与EMT有关。
Objective： This study sought to investigate whether n-butanol part of Pterocephalus hookeri （YSC-ZDC） in vitro inhibits the invasion and metastasis of human liver cancer Hep3B cells effective extraction, and its antitumor mechanisms were discussed. Method： Hep3B cell proliferation was assayed by MTT method, the fluorescent staining was used to observe the effect of YSC-ZDC on Hep3B cell apoptosis. Scratch test and Transwell were used to detect invasion and migration of Hep3B cell by YSC-ZDC, Western blot was uswed to detect related signaling pathways. Result： YSC-ZDC 50, 100, 200 mg ·L^-1 24, 48, 72 h after treatment could obviously inhibit proliferation. 50, 100, 200 mg ·L^-1 YSC-ZDC after 24 h could obviously inhibit the invasion and migration; YSC-ZDC could significantly affect the epitheliai-mesenchymel transitions （EMT） related proteins. Conclusion： YSC-ZDC can significantly inhibit Hep3B ceils proliferation and invasion and metastasis, and induce the Hep3B cell apoptosis. Its mechanism may be related to EMT.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第3期100-105,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药管理局项目(A1-Z1401010504)
上海市博士点基金(K130204)
关键词
翼首草
HEP3B细胞
侵袭
转移
上皮-间质转化
Pterocephalus hookeri
Hep3B cells
invasion
metastasis
epithelial-mesenchymel transitions
