摘要
目的:建立猴耳环叶的HPLC指纹图谱。方法:色谱条件为Odyssll C18色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-0.5%磷酸水梯度洗脱,柱温35℃,流速1 m L·min-1,检测波长273 nm,进样量10μL。结果:采用UPLC/DAD/Q-TOFMS xevo G2 Q-Tof LC-MS对15个共有峰中的6个色谱峰进行初步归属,确定为没食子酸、木犀草苷、没食子酸乙酯、(-)-(2S)-5,3',4',5'-四羟基黄烷-7-没食子酸酯和7,4'-二-O-没食子酰基特利色黄烷、槲皮苷,10批猴耳环叶的相似度均在0.97以上。结论:该方法符合方法学要求,可用于猴耳环叶的全面质量评价。
Objective： To establish the HPLC fingerprint of Archidendron clypearia leaves. Method： The chromatographic fingerprint was obtained with Odyssll Cl8 column （4.6 mm × 250 mm, 5μm） and gradient eluted with methanol and 0.5% phosphoric acid. The column temperature was maintained at 35 ℃ , and the flow rate was 1 mL · min^-1 The detection wavelength was 273 nm. The injection volume was 10 μL. Result： Six chromatographic peaks among 15 common peaks were identified by UPLC/DAD/Q-TOF-MS xevo G2 Q-Tof LC-MS and be intended to be gallic acid, galuteolin, gallic acid ethyl ester, （-） -（2S） -5, 3＇, 4＇, 5＇- tetrahydroxyflavan-7-gallate, 7, 4＇-O-di-gallyohricetiflavan, quercitrin. The similarities of ten batches of Archidendron clypearia leaves were above 0.97. Conclusion： The method accordance with the requirements of the methodology and can be used for the full quality evaluation of Archidendron clypearia leaves.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第3期63-66,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
猴耳环
高效液相色谱法
指纹图谱
Archidendron clypearia leaves
HPLC
fingerprint spectrum
