摘要
目的:优选龙葵总多糖的闪式提取工艺,为该药材的资源开发提供参考。方法:采用苯酚-硫酸法测定总多糖含量,检测波长490 nm。以总多糖提取量和浸膏得率为综合评价指标,采用正交试验考察提取电压、提取时间、提取次数、料液比对龙葵总多糖闪式提取工艺的影响,并与热水回流法和超声法进行比较。结果:最佳提取工艺为加30倍量水于80 V提取2次,每次2 min。热水回流法、超声法及闪式提取法的总多糖提取量分别为3.760,3.232,4.585 mg·g-1。结论:闪式提取法的提取效率显著高于其他2种提取方式,适用于龙葵总多糖的提取,该工艺简单、迅速,具有良好的应用前景。
Objective： To optimize flash extraction process of total polysaccharides from Solanum nigrum. Method： Phenol-sulfuric acid method was used to determine the content of total polysaccharides with detection wavelength at 490 rim. With extracting amount of total polysaccharides and yield of extract as comprehensive index, effects of extraction voltage, extraction time, extraction times and solid-liquid ratio on flash extraction process were investigated by orthogonal test. Optimum flash extraction process was compared with hot reflux method and ultrasonic method. Result： Optimum flash extraction process was adding 30 times the amount of water at 80 V and extracting 2 times for 2 min of each time. Extracting amounts of total polysaccharides were 3. 760, 3. 232, 4. 585 mg ·g^-1 by hot reflux extraction, ultrasonic and flash extraction method, respectively. Conclusion： Extraction efficiency of flash extraction method is significantly higher than the other two kinds of methods, which is suitable for extracting total polysaccharide from S. nigrum, it is feasible and rapid with good application prospects.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第3期24-26,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
浙江省教育厅科研项目(Y201431468)
嘉兴市科技局科技项目(2009AY2067)
关键词
龙葵
多糖类
闪式提取法
浸膏得率
Solarium nigrum
total polysaccharides
flash extraction method
extract yield
