摘要
采用高效液相色谱法对山西省5地6处野生葛根中主要有效成分葛根素、大豆苷及总黄酮含量进行了分析,并对HPLC法的测定条件（流动相和洗脱程序）进行了优化。结果表明,6种晋产野生葛根中主要有效成分含量差异显著,葛根素的含量为1.46%~4.48%,大豆苷的含量为0.11%~0.62%,总黄酮的含量为11.19%~26.14%。葛根素、总黄酮含量与纬度高低呈正相关。晋南绛县么里镇小北山与夏县泗交镇太宽河自然保护区的野生葛根中葛根素含量低于《中国药典》规定的2.4%,而晋北灵丘县的野生葛根中葛根素含量则高达4.48%。
In this study,the content of main effective ingredients including puerarin,daidzin and total flavonoids of Pueraria lobata from 5 areas of Shanxi were investigated by HPLC. In addition,the HPLC analysis conditions were optimized.The results showed that： there was a significant difference in the content of puerarin,daidzin and total flavonoids of 6samples of P. lobata. The contents of puerarin,daidzin and total flavonoids were 1. 46% ~ 4. 48%,0. 11% ~ 0. 62% and11. 19% ~ 26. 14%,respectively. Puerarin and total flavonoids had positive correlation with the cultivation latitude of P.lobata,The contents of puerarin in P. lobata samples from Xiaobei Mountain,Meli town,Jiangxian and Taikuan river Nature Reserve,Sijiao town,Xiaxian were not match up with the standard（2. 4%） required by Chinese Pharmacopoeia,while the content of puerarin from Panjiapu,Xiaguan,Lingqiu was determined to be 4. 48%.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第1期99-102,133,共5页
Natural Product Research and Development
基金
国家"十二五"科技支撑计划项目(2011BA107B05-4)
关键词
葛
种质资源
葛根素
大豆苷
总黄酮
Pueraria lobata
germplasm resources
daidzin
puerarin
total flavonoids
