摘要
研究了洋山航道工程疏浚泥悬浮液和浸出液对三疣梭子蟹(Portunus trituberculatus)大眼幼体的毒害效应,结果表明:(1)大眼幼体死亡率随着悬浮液和浸出液配比浓度的增大而逐渐增加。浸出液对大眼幼体的96 h半致死配比浓度φ(LC50)为1 294.66 m L·L-1,安全配比浓度φ(SC)为129.466 m L·L-1;悬浮液对大眼幼体的96hφ(LC50)为996.17 m L·L-1,φ(SC)为99.617 m L·L-1。(2)大眼幼体的蜕皮率随着悬浮液和浸出液配比浓度的增加而逐渐降低。同体积配比浓度下,浸出液处理组蜕皮率略高于悬浮液处理组。悬浮液抑制三疣梭子蟹大眼幼体的蜕壳,而浸出液抑制新壳的硬化。(3)浸出液和悬浮液胁迫24 h后,大眼幼体肌肉中HSP70表达量迅速升高,浸出液和悬浮液最高配比浓度组表达量显著高于对照组(P<0.05);72 h各处理组HSP70表达量均达到最高值;96 h后各处理组表达量有所降低,最高配比浓度组HSP70表达量降幅明显(P<0.05),对照组表达量仍继续上升。
Toxic effect of suspension and lixivium from the dredged sludge of the Yangshan channel project on megalopa of Portunus trituberculatus was evaluated, through a laboratory simulation experiment. Results show that ( 1) mortality of the megalopa increased gradually with concentration of the suspension and lixivium, and 96 h LC50 and SC of the lixivium to megalopa of Portunus trituberculatus was 1 294?66 and 129?466 mL·L-1, respectively, and of the suspension, 996?17 and 99?617 mL·L-1 , respectively;( 2) molting rate of the megalopa decreased with increasing concentration of the sus?pension and lixivium;it was slightly higher in the group of lixivium treatments than in the group of suspension treatments group when they were the same in volume concentration;the suspension inhibited molting of megalopa, while the lixivium inhibited hardening of new shell;( 3) After 24 hours of exposure to the suspension and lixivium, the expression of HSP70 in the muscle of megalopa increased rapidly; after 72 hours, it peaked in all the treatments; and after 96 hours, it de?creased to a varying extent in all the treatments and the most significantly in the treatment the highest in concentration;while in the control it kept on rising.
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2015年第1期118-123,共6页
Journal of Ecology and Rural Environment
基金
上海市科学技术委员会项目(12231203403)
国家重点基础研究发展计划(G2010CB429005)
关键词
三疣梭子蟹
疏浚泥
蜕皮率
HSP70
suspension
lixivium
Portunus trituberculatus
molting rate
HSP70
