摘要
目的:建立并比较两面针与毛两面针的高效液相色谱指纹图谱。方法:采用高效液相色谱法。色谱柱为Promosil C18,流动相为乙腈(流动相A)-0.2%磷酸-0.2%三乙胺水溶液(流动相B)(梯度洗脱),流速为1.0 ml/min,检测波长为283 nm,柱温为20℃,进样量为10μl。收集不同批次的33批样品进行测定,并使用聚类分析和主成分分析对指纹图谱进行模式识别研究,利用《中药色谱指纹图谱相似度评价系统》(2004A版)对33批样品的相似度进行评价。结果:从两面针、毛两面针药材的指纹图谱中分别得出18、27个特征峰,经对照,指认出木兰花碱、橙皮苷、氯化两面针碱、乙氧基白屈菜红碱4种共有成分,而毛两面针素为毛两面针药材独有,两种药材的相似度均>0.87。两者除指标成分的种类不同外,其含量也有较明显的差异。结论:两面针与毛两面针药材所含指标成分的种类和含量均有所不同,该方法可为两面针和毛两面针的鉴别提供依据。
OBJECTIVE： To compare and establish HPLC fingerprint of Zanthoxylum nitidum （Roxb.） DC and Z. nitidum f. fastuosum. METHODS：HPLC method was adopted. The determination was performed on Promosil C,, column with mobile phase consisted of acetonitrile （A）-0.2% phosphoric acid-0.2% triethylamine solution （B） with gradient elution at the flow rate of 1.0 ml/ min. The detection wavelength was set at 283 nm and column temperature was 20 ℃. The sample size was 10 μl. 33 samples from various batches were analyzed, and cluster analysis and principal component analysis （PCA） were applied to study fingerprint and chemical pattern recognition. The similarity of 33 batches of samples was evaluated by Important Chromatogram Fingerprint Similarity Evaluation System （2004 A edition）. RESULTS： 18 and 27 characteristic peaks were identified in the fingerprint of Z. nitidum （Roxb.） DC and Z. nitidum f. fastuosum. 4 common components were identified, as magnoflorine, hesperidin, nitidine chloride and ethoxychelerythrine. Toddalolactone was only found in Z nitidum f. fastuosum. The similarity of two medicinal materials were higher than 0.87. The result showed that their contents of samples were different significantly and the types of indicator components were different. CONCLUSIONS： The indicator components of Z. nitidum （Roxb.） DC and Z. nitidum f. fastuosum are different in type and content. The method is stable, precise and reproducible, and can be used for the identification of Z. nitidum （Roxb.） DC and Z nitidum f. fastuosum.
出处
《中国药房》
CAS
北大核心
2015年第3期377-380,共4页
China Pharmacy
基金
湖南省中医药科研计划项目(No.2009008)
长沙市科技计划项目(No.K0802115-31)
关键词
两面针
毛两面针
聚类分析
指纹图谱
高效液相色谱法
对比
Zanthoxylum nitidum （Roxb.） DC
Z. nitidum f. fastuosum
Cluster analysis
Fingerprint
HPLC
Comparison
