摘要
胶内酶切是蛋白质组研究中衔接电泳分离和质谱鉴定的重要环节,对最终的蛋白质定性和定量分析结果有显著的影响。该技术自1992年初步建立以来,一直处于不断完善中,出现了种类繁多的改进方案。为了更有效地利用胶内酶切技术,从凝胶脱色、杂质去除、蛋白酶切、肽段提取4个方面归纳整理了近年来蛋白质胶内酶切技术的主要研究进展。
In-gel digestion is the important step that connects electrophoresis separation and mass spectrometry identification in proteome research, and can significantly affect the results of qualitative and quantitative analysis of proteins. The technology was firstly proposed in 1992, and from then on, many progresses have been made and various improvement plans have been developed. In order to more effectively use the technology of in-gel digestion, the progress on the aspects of gel decoloration, impurity removal, protein digestion and peptides extraction were reviewed from the literature.
出处
《生物技术通报》
CAS
CSCD
北大核心
2015年第1期61-66,共6页
Biotechnology Bulletin
基金
国家“863”计划项目(2008AA10Z115)
国家自然科学基金项目(30971874)
转基因生物新品种培育科技重大专项(2009ZX-08012-011B)
中国农业科学院科技创新工程(作物分子标记技术及其应用科研创新团队)
关键词
蛋白质胶内酶切
样品制备
质谱分析
电泳分离
技术进展
in-gel protein digestion
sample preparation
mass spectrometry
electrophoretic separation
technical progress
