摘要
目的：建立同时测定更年舒丸中没食子酸、马钱苷、葛根素、阿魏酸、大黄素、大黄素甲醚含量的方法.方法：采用HPLC测定,Agilent C18色谱柱（ZORBAX,SB-AQ,4.6 mm ×250 mm,5μm）,流动相乙腈-0.2％甲酸水,梯度洗脱,流速1.0 mL·min^-1,柱温35℃,检测波长238 nm（马钱苷）,270 nm（没食子酸、葛根素、大黄素、大黄素甲醚）,320 nm（阿魏酸）.结果：6个成分的方法学考察均合格,阴性均无干扰,平均加样回收率为98.2％ ～ 103.2％,RSD 1.4％ ～ 1.9％.结论：该方法简便、准确、专属性强,为更年舒丸及其类似组方制剂的质量评价提供了研究基础.
Objective：To establish an HPLC method for determination of gallic acid,loganin,puerarin,ferulic acid,emodin and physcion in Gengnianshu pills.Method：HPLC was applied on an Agilent C18 column （Zorbax SB-AQ 4.6mm × 250 mm,5 μm） with acetonitrile-0.2% formic acid water as mobile phase by gradient elution.The flow rate was 1.0 mL ·min^-1.Detection wavelengthes were set at 238 nm （loganin）,270 nm （gallic acid,puerarin,emodin and physcion）,and 320 nm （ferulic acid）.Result：The methodology of 6 components met the requirements,the blank test showed no interference,the average recovery rates were 98.2%-103.2%,and RSD was 1.4%-1.9%.Conclusion：This method is easy,accurate with high specificity.This study provides scientific basis for the quality evaluation of Gennianshu pills.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第2期83-86,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河南省科技攻关项目(0524420029)
