摘要
目的：探讨β2-肾上腺素受体（β2-AR）基因Gln27Glu多态性与小儿支气管哮喘风险之间的关系。方法：采用聚合酶链反应-限制性内切酶片段长度多态性（RFLP）和等位基因频率分析法对120例支气管哮喘患儿进行β2-AR基因Gln27Glu多态性检测,并对相关因素做Logitics回归分析。结果：哮喘组Gln/Glu基因型比例最高（56.67%）,对照组Gln/Gln比例最高（49.57%）（P〈0.05）。纯合子Glu/Glu发生哮喘风险最高（OR=2.171,95%CI=0.237-23.140）,Glu等位基因频率明显高于对照组（P〈0.05）。结论：β2-AR基因以杂合子Gln/Glu基因型为主,Glu与哮喘危险性相关,但不是哮喘发病的独立危险因素。
Objective To explore the correlation between genetic polymorphisms of β2-adrenergic Gln27Glu and bronchial asthma in child. Methods The polymorphisms of β2-adrenergic receptors（β2-AR） Gln27Glu in 120 children with bronchial asthma were detected with restriction fragment length polymorphism（RFLP） ,Allele frequency analysis method and multivariate logistic regression analysis were also conducted. Results The frequency of Gln/Glu genotype of β2-AR 27 loci was the highest in asthmatic group （56. 67% ）, while the frequency of Gln/Gln genotype was the highest in control group （49. 57% ）. The results of odds ratio demonstrated that the risk of asthma with Glu/Glu homozygote was the highest（ OR = 2.171,95% CI = 0.237 - 23. 140）, and the frequency of Glu allele in asthmatic group was significantly higher than that of control group（P 〈0.05）. Conclusion The main genotype of β2-AR was Gln/Glu heterozygote,the Glu may not be independent risk factor ,but associated with asthma.
出处
《湖北医药学院学报》
CAS
2014年第6期552-555,560,共5页
Journal of Hubei University of Medicine
