摘要
目的观察补阳还五汤对大鼠急性脊髓损伤(ASCI)的治疗作用及其机制。方法 SD大鼠随机分为空白组、损伤对照组、补阳还五汤组,各10只。采用Allen’s打击法建立急性脊髓损伤模型,空白组和损伤对照组造模后予蒸馏水1m L灌胃,补阳还五汤组造模后予补阳还五汤1m L灌胃,均1天1次,连续3天。各组分别于术后1、7、14天观察其行为学BBB评分,14天通过免疫组化的方法观察脊髓损伤部位神经细胞凋亡(caspase-3p20、Bcl-2与Bax)、血管内皮生长因子(VEGF)的表达水平。结果 1行为学观察评分:术后7、14天补阳还五汤组与损伤对照组下肢功能均有恢复(P<0.05),且补阳还五汤组较损伤对照组恢复更明显[(9.05±0.95)分比(5.75±1.97)分,(12.6±1.55)分比(8.80±3.34)分,P<0.05]。2免疫组化检测:补阳还五汤组caspsase-3p20阳性细胞数表达较损伤对照组少[(16.70±2.21)个比(20.30±4.13)个,P<0.05];补阳还五汤组Bcl-2阳性细胞的表达较损伤对照组增加[(10.57±0.97)个比(6.42±3.20)个,P<0.05];补阳还五汤组Bax阳性细胞的表达较损伤对照组少[(18.29±2.21)个比(21.14±2.41)个,P<0.05];补阳还五汤组VEGF阳性细胞的表达较损伤对照组多[(11.75±1.03)个比(9.50±1.30)个,P<0.05]。结论补阳还五汤可提高脊髓损伤部位VEGF的表达,使细胞凋亡明显减少,促进骨髓功能的恢复。
Objective To determine the therapeutic efficacy of Buyang Huanwu Decoction（BYHWD） in the treatment of acute spinal cord injury（ASCI） in rats, and to investigate the underlying mechanism. Methods Thirty SD rats were randomized into control group（laminectomy only）, ASCI group, ASCI＋BYHWD group, 10 rats in each group. ASCI was induced by using the modified weight-drop method in the latter two groups. Rats in control and ASCI groups were intragastrically given distilled water once daily for 3d,rats in ASCI ＋BYHWD group were given BYHWD for the same period. Locomotors functional recovery was assessed on day 1,7,14 after operation by performing open-field locomotors tests（BBB score）. On postoperative day 14,the segments of spinal cord encompassing the injury site were removed for histopathological analysis. Immunohistochemistry was performed to observe the expression of B-cell CLL/lymphoma-2（Bcl-2）,BCL-2-associated X protein（Bax）,caspase-3,and vascular endothelial growth factor（VEGF）. Results Both of the BYHWD-treated rats and ASCI rats had locomotor function recovery on postoperative day 7 and 14（P〈0.05）,but the BYHWD-treated rats had significantly better locomotor function recovery than ASCI group did（D7：9.05±0.95 vs 5.75±1.97;D14：12.60±1.55 vs 8.80±3.34;all P〈0.05）. Compared with ASCI group,the BYHWD-treated rats had reduced expression of caspase-3（16.70±2.21 vs 20.30±4.13,P〈0.05） and Bax（18.29±2.21 vs 21.14±2.41,P〈0.05）,increased expression of Bcl-2（10.57±0.97 vs 6.42±3.20,P〈0.05） and VEGF（11.75±1.03 vs 9.50±1.30,P〈0.05）. Conclusion BYHWD treatment after ASCI can significantly improve locomotor recovery by upregulating VEGF and inhibiting neural apoptosis.
出处
《浙江中西医结合杂志》
2015年第1期8-11,I0002,共5页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省中医药科技计划项目(No.2010ZB049)
浙江省教育厅科研项目(No.Y200906743)
