摘要
目的初步了解大鼠血浆和胃底组织中ghrelin的分泌规律。方法 56只雄性SD大鼠,按体重随机分配到自由摄食组、禁食组及餐后0、30、60、90、120min各时间点组。采用ELISA法检测血浆和胃底组织中ghrelin含量的变化。结果大鼠血浆和胃底组织中ghrelin水平在禁食后有升高的趋势。餐后血浆中ghrelin逐渐升高,第90min突然降低,第120min又有所回升。而胃底组织中ghrelin的变化趋势恰好与血浆相反。结论 ghrelin与摄食密切相关,验证了ghrelin是刺激食欲的一种生理因素。同时结果提示,ghrelin在血浆和胃底组织可能存在相反的变化趋势。
Objective To detect circulating and gastric fund＇s ghrelin levels in male SD rats after fasting,fasting followed by re-feeding.Methods Totally 56 male SD rats were randomly assigned into 7groups including ad libitum fed group,fasted group and 5 fasted -refed groups.Rats in the ad libitum fed and fasted group were anaesthetized at 7：00,and different cohorts in the refed groups were anaesthetized every 30min beginning at 7：30.The plasma and gastric fund＇s ghrelin level was detected with ELISA kit.Results Both plasma and gastric fund＇s ghrelin level increased after fasting.Plasma ghrelin level decreased just after food stimulus finished,but recovered at 90min,decreased again at 120min.Interestingly gastric fund＇s ghrelin level changed reversedly.Conclusion Ghrelin play a very important role in appetite regulation.
出处
《医学研究杂志》
2014年第12期136-139,共4页
Journal of Medical Research
