摘要
目的:应用小鼠Znf230多克隆抗血清,对Znf230在小鼠睾丸及精子中的表达及定位进行研究。方法:用RT-PCR分析、Western blot检测、免疫组织化学和免疫荧光染色方法研究Znf230在小鼠不同发育阶段睾丸组织及精子中的表达及亚细胞定位情况。结果:小鼠从出生到成年的发育过程中,Znf230的表达水平基本上是稳定的。小鼠出生6 d和12 d时,该蛋白在精原细胞核中表达,从出生18 d到精子发育成熟这个阶段,该蛋白转移到圆形精子细胞、延长精子细胞和精子中表达,具体定位于顶体区域和精子的尾部。结论:Znf230可能在小鼠生精过程中起着重要的作用。
Objective：To detect the expression and location of Znf230 in mouse testis and spermatozoa by Znf230 polyclonal antiserum. Methods ： Expression and localization of Znf230 in mouse testis at different developmental stages and epididymal spermatozoa were determined by RT-PCR,immunoblotting, immunohistochemistry and immunoluorescence. Results： Znf230 remained relatively stable and was expressed throughout postnatal development of the mouse testis. It was primarily expressed in the nuclei of spermatogonia（from postnatal d 6 to postnatal d 12） and subsequently in the acrosome system and the entire tail of round spermatids,elongated spermatids and spermatozoa（from postnatal d 18 to mature sperm）. Conclusion：Znf230 may play an important role in mouse sper matogenesis.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2014年第11期1552-1555,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金(编号:30470960)
国家重点基础研究发展计划(973计划)(编号:2004CB518805)
关键词
Znf230
表达
定位
生精相关基因
Znf230
expression
localization
spermatogenesis-related gene
