摘要
目的:探讨交通心肾法中药对老龄雌鼠下丘脑-垂体-卵巢雌激素受体(estrogen receptor,ER)的影响。方法:选用15月龄雌性SD大鼠,将其分为空白组、中药治疗组、西药对照组和中药对照组,分别给予生理盐水、宁更丹、戊酸雌二醇和更年安灌胃,另选3月龄大鼠作为青年对照组,采用酶免法检测大鼠下丘脑、垂体、卵巢ER。结果:与空白组相比,中药治疗组下丘脑、垂体和卵巢的雌激素受体含量均明显升高,有显著性差异(P<0.05)。中药对照组和西药对照组仅能升高下丘脑的ER,有显著性差异(P<0.05)。结论:交通心肾法中药能升高下丘脑、垂体和卵巢的雌激素受体含量,可能是其治疗更年期综合征的作用机制之一。
Objective： To study the effect of herbs of Jiaotong Xinshen therapy on hypothalamus-pituitary-ovarian ER in old female rats. Methods： Choose female SD rats aged 15 months,and they were divided into blank group,Chinese medicine treatment group,western medicine control group,Chinese medicine control group. They were given normal saline,Ning Gengdan,progynova and Gengnian ＇an gavage treatment,and choose three-month old rats as the young control group. Elisa method was used to measure the levels of hypothalamus,pituitary and ovarian ER. Results： Compared with the blank group,the hypothalamus,pituitary and ovarian estrogen receptor levels in Chinese medicine treatment group were significantly elevated,and there were significant differences（P〈0. 05）. Traditional Chinese medicine control group and western medicine control group could only increase the hypothalamus ER,and there were significant differences（P〈0. 05）. Conclusion： The therapy of Jiaotong Xinshen can increase the hypothalamus,pituitary and ovarian ER levels,which may be one of the mechanism in the treatment of menopausal syndrome.
出处
《中医药学报》
CAS
2014年第6期45-47,共3页
Acta Chinese Medicine and Pharmacology
基金
北京市自然科学基金(7112070)
北京中医药大学证候规范化标准化创新团队基金(2011-CXTD-08)
