摘要
【目的】为了分析我国不同地区禾谷缢管蚜Rhopalosiphum padi(Linnaeus)田间种群Ace1基因的选择性剪切、Ace1基因选择性剪切频率及Ace1基因选择性剪切对其ACh E1结构的影响。【方法】从我国11个省(市)的16个地区采集田间试虫,通过RT-PCR技术,克隆各个地区及室内敏感品系各15头禾谷缢管蚜试虫的Ace1基因序列的全长,分析并鉴定Ace1的选择性剪接,在SABLE服务器和I-TASSER服务器上分别分析选择性剪切对ACh E1的二级结构和三级结构的影响。【结果】16个地理种群中,9个地理种群都存在选择性剪切,不同地理种群该选择性剪切的频率不同;剪切位点靠近Ace1基因的5′端,其对应的核苷酸序列为5′-ATCCGAATTTAG-3′,导致4个氨基酸(RSEF)缺失;选择性剪切改变了局部的二级结构,并在一定程度上改变其三级结构。【结论】禾谷缢管蚜田间种群中较为普遍地存在一个Ace1基因的选择性剪切,该Ace1基因选择性剪切改变了ACh E1的空间结构,并可能改变ACh E1的代谢特性。
[Objectives] Acetylcbolinesterase （ACHE） plays a vital role in the nervous system of insects and serves as the target for various organophosphate and carbamate insecticides. The aims of this study were to identify alternative splicing of the Ace1 gene in R. padi field populations sampled in different regions of China and the effect of alternative splicing on the secondary and tertiary structure of ACHE1. [Methods] R. padi field populations were collected from 16 different regions of 11 Chinese provinces. RT-PCR was used to clone the cDNA sequences of the Ace1 gene from 15 individuals from each field population and a susceptible laboratory strain. The deduced amino acid sequences of R. padi ACHE1 were submitted to the SABLE online and I-TASSER online servers to analyze the effect of alternative splicing on the secondary and tertiary structure of ACHE1. [Results] Alternative splicing was found in individuals from nine field populations. This occurred near the 5＇ end of the ACHE1 coding region, resulting in the deletion of ＂ATCCGAATTTAG＂ and the amino acids ＂RSEF＂. Results of structural analysis showed that alternative splicing changed the secondary structure and tertiary structure of ACHE1. [Conclusion] Alternative splicing is common in wild populations of R. padi. This can change the spatial structure and catalytic properties of AChE 1.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2014年第6期1488-1495,共8页
Chinese Journal of Applied Entomology
基金
国家自然科学基金(31272036,31471766)
教育部高等学校博士学科点专项科研基金(20110204110001)
关键词
禾谷缢管蚜
乙酰胆碱酯酶
选择性剪切
选择性剪切频率
三级结构
Rhopalosiphum padi, acetylcholinesterase, alternative splicing, frequency of alternative splicing, tertiary structure
