摘要
目的 旨在检测选择性线粒体分裂抑制剂-1（Mdivi-1）预处理对大鼠急性脊髓损伤（ASCI）后线粒体形态,线粒体膜电位（MMP）及线粒体ATP含量的影响。方法 成年雌性SD大鼠72只,体重250~300 g,采用随机数字表法,将大鼠随机分为3组（n=24）：假手术组（Sham组）、单纯脊髓损伤组（SCI组）和Mdivi-1预处理组（1.20 mg/kg,Mdivi-1组）,每组24只。SCI组和Mdivi-1组大鼠采用Allen’s方法制备ASCI模型,Mdi-vi-1组和SCI组大鼠在脊髓打击之前15 min经尾静脉分别给予Mdivi-1或等容量二甲基亚砜（DMSO）。每组大鼠再分为两个亚组,分别于脊髓暴露或者打击后的3和12 h处死,取出脊髓T9-11,采用透射电子显微镜检测线粒体形态,用荧光显微镜和荧光分光光度计检测MMP变化,用荧光分光度计检测线粒体ATP含量的变化。结果 与Sham组相比,SCI 3 h组时,线粒体数目明显减少（〈0.01）,截面积明显增大（〈0.01）,但MMP和线粒体ATP含量无明显变化;SCI 12 h组时,线粒体数目明显增多（〈0.01）,截面积明显减小（〈0.01）,但MMP和线粒体ATP含量明显减低（〈0.01）。与SCI组相比,Mdivi-1 3 h组时,线粒体数目、截面积及MMP和线粒体ATP含量无明显变化;而Mdivi-1 12 h组时,线粒体数目明显减少（〈0.01）,截面积明显增大（〈0.01）,MMP和线粒体ATP含量明显升高（〈0.01）。结论 选择性线粒体分裂抑制剂-Mdivi-1能有效抑制ASCI后线粒体分裂和MMP降低,增加线粒体中ATP的合成,从而保护了线粒体功能。
[Objective] To detect the effect of selective mitochondrial fission inhibitor-Mdivi-1 on mitochondrial morphology, mitochondrial membrane potential and the content of mitochondrial ATP after acute spinal cord injury in rats. [Methods] 72 adult female SD rats , weighing 250-300 g, were randomly divided into 3 groups （n =24）： sham operation group（Sham group）, acute spinal cord injury group （SCI group）, Mdivi-1 pretreatment group （1.20 rag/ kg, Mdivi-1 group） using a random number table. The Spinal cord injury model of rats in SCI group and Mdivi-1 group were made by Allen＇s method. In sham group, the rats were exposed, but not hit. In Mdivi-1 group, the rats were given Mdivi-1 15 rain before spinal cord injury through the tail vein, but SCI group received the same amount of dimethyl sulfoxide（DMSO）. Each group was divided into two sub-groups. The rats were sacrificed at 3 h and 12 h respectively after exposing spinal cord or spinal cord injury, and then the spinal cord T9-11 was removed. Mitochon- drial morphology was detected by transmission electron microscopy. Mitochondrial membrane potential was detected by fluorescence microscope and fluorescence spectrophotometer. The content of mitochondrial ATP was detected by fluorescence spectrophotometer. [ Results ] Compared with Sham group, the number of mitochondria in SC1 3 h group reduced significantly （P 〈0.01） and cross-sectional area increased significantly （P 〈0.01）, but no significant changes were found in mitoehondrial membrane potential and content of mitochondrial ATP; The number of mitochondria in the SCI group at 12 h increased significantly （P 〈0.01）, but cross-sectional area, mitochondrial membrane potential and content of mitochondrial ATP reduced significantly （P 〈0.01）. Compared with the SCI group, the number of mi- toehondria, cross-sectional area, mitochondrial membrane potential and content of mitochondrial ATP have no slgnif~ icant changes in the Mdivi-1 group at 3 h; However, the number of mitochond
出处
《中国现代医学杂志》
CAS
北大核心
2015年第1期1-6,共6页
China Journal of Modern Medicine
基金
国家自然科学基金(No:81272074)
辽宁省博士科研启动基金(No:20121094)
辽宁医学院校长基金(No:XZJJ20130204)
关键词
急性脊髓损伤
线粒体分裂抑制剂
线粒体形态
线粒体膜电位
三磷酸腺苷
acute spinal eord injury
mitochondrial fission inhibitor
mitochondrial morphology
mitochondrial membrane potential
adenosine triphosphate
