摘要
建立了鱼糜中阿苯达唑及其代谢物阿苯达唑亚砜、阿苯达唑砜和阿苯达唑-2-氨基砜多残留同时检测的超高效液相色谱-串联质谱联用（UPLC-MS/MS）方法.鱼糜样品经乙酸乙酯提取,正己烷净化,超高效液相色谱-串联质谱法检测.本方法中阿苯达唑定量限为0.5 μg/kg,线性范围为0.5 ～ 50ng/mL;阿苯达唑亚砜、阿苯达唑砜和阿苯达唑-2-氨基砜的定量限均为0.1 μg/kg,线性范围为0.1-～ 10ng/mL.在加标水平为0.5～5.0μg/kg时阿苯达唑的平均回收率为96.6％ ～ 99.7％,RSD为5.95％ ～9.95％;在加标水平为0.1～1.0μg,/kg时,阿苯达唑代谢物的平均回收率为90.8％ ～105.6％,RSD为2.16％ ～9.15％.
A method for determination of albendzole （ABZ） and its metabolites, albendazole sulfoxide （ABZSO） , albendazole sulfone （ ABZSO2 ） and albendazole aminosulfone （ ABZ - 2 - NH2 - SO2 ） in surimi with ultra performance liquid chromatography - tandem mass spectrometry （ UPLC - MS/MS） was established. The surimi samples were extracted by ethyl acetate and cleaned up by n -hexane, and detected by LC - MS/MS. The limit of quantitation （LOQ） of ABZ was 0.5 pug/kg, and the linear range was 0.5 -50 ng/mL, while the LOQ of its metabolites was 0.11xg/kg, and the linear range was 0.1 - 10 ng/ mL. When the standard addition level was 0.5 - 51xg/kg, the average recoveries of ABZ was 96.6% - 99.7% ,and the RSD was 5.95% -9.95%. When the standard addition level was 0.1 - 11xg/kg, the average recoveries of its metabolites was 90.8% - 105.6% ,and the RSD was 2.16% -9.15%.
出处
《肉类工业》
2014年第8期40-43,共4页
Meat Industry
关键词
阿苯达唑及其代谢物
超高效液相色谱-串联质谱联用
鱼糜
albendzole and its metabolites
ultra performance liquid chromatography tandem mass spectrometry
surimi
