摘要
采用日本乙型脑炎病毒单克隆抗体预包被酶标板，将纯化的日本乙型脑炎病毒（ JEV）作为检测用抗原，利用包被捕获法建立了用于检测猪乙型脑炎抗体的间接ELISA法。采用建立的ELISA法对50份已知阴性血清样本检测，临界OD450nm值为0．343，ELISA与IFA对200份血清进行平行检测，总符合率为92．9％，与商品化的同类国产试剂盒的符合率为95％。与其他常见的猪病毒阳性血清抗体无交叉反应，2～8℃保存12个月稳定。研制的ELISA抗体检测试剂盒为临床JEV血清抗体检测及其疫苗的免疫效果评价提供了技术手段。
With capture coating method, indirect ELISA method for detecting Japanese encephlitis antibody was established using Japanese encephlitis virus monoclonal antibody to pre-coat ELISA plate and using purified Japanese encephlitis as detecting antigen. The ELISA was optimized and the kit was developed for checking the immune results of JEV vaccines. In comparison with inderect immunofluorescence （ IFA ） , there was 92. 9%coincidence between the ELISA and IFA based on detection of 200 serum samples. With the commercialization of the same kind of domestic kits,there was 95% coincidence.There was no cross reactions for the positive serums of other common Japanese encephlitis antibodies.The kit was stable kept up to 12 months at 2~8℃.The kit was used for clinical laboratory and detection of serum antibody.
出处
《中国兽药杂志》
北大核心
2015年第1期10-13,共4页
Chinese Journal of Veterinary Drug
