摘要
腺相关病毒是动物病毒中最小,基因结构最简单的一类单链DNA缺陷病毒。其最早于1965年作为腺病毒制备物中的一种污染成分被发现。在培养的正常细胞中,单纯的腺相关病毒并不发生毒性感染,只有在与其他辅助病毒发生共同感染时才能产生作用。由于安全性好、宿主范围广、免疫原性低等特点,腺相关病毒被广泛应用于基因表达载体的构建及基因治疗。腺相关病毒的随机整合性仍然可能引起临床应用上的潜在危险。在阐述该病毒的分子结构及其在生物学上的特点基础上,说明其在实际生产中的运用,并对其有关研究进行了展望。
Adeno-associated virus （AAV） is the smallest defective animal virus with a simple gene structure and a single stranded DNA. As early as 1965, AAV had been found as a kind of pollution components in the adenovirus preparation. AAV can’t infect normal cells alone in culture, and can only have a common infection with other helper virus. AAV has been widely used in the construction of gene expression and gene therapy for its safety, board host and low immunogenicity. Random integration of AAV may cause a potential risk for clinical application. In the view, we will elaborate the molecular structure and biological characteristics of the virus. At the same time, we will also illustrate its application in production.
出处
《生物灾害科学》
2014年第4期333-336,共4页
Biological Disaster Science
基金
国家自然科学基金资助项目(30970880)
