摘要
为探讨凝集素提取液对刺参Apostichopus japonicus免疫的影响,将刺参基础饲料中分别添加质量分数为0.64%、1.28%、2.56%的海带凝集素提取液,或质量分数为1.88%、3.76%、7.52%的条斑紫菜凝集素提取液,分别投喂6个试验组刺参(体质量为3.50 g"1.95 g),以基础饲料饲养组为对照,分别于投喂凝集素提取液后第4、8、12、17天时,检测各组刺参体壁中过氧化氢酶(CAT)、溶菌酶(LSZ)、碱性磷酸酶(AKP)和酸性磷酸酶(ACP)的活性。结果表明:投喂海带凝集素提取液后,各浓度组刺参CAT、LSZ活性在第8、12、17天时均有显著或极显著提高(P<0.05或P<0.01),0.64%组ACP活性在第8、17天时有显著提高(P<0.05);投喂条斑紫菜凝集素提取液后,各浓度组刺参CAT活性在第8、12、17天时有显著或极显著提高(P<0.05或P<0.01),7.52%组LSZ活性在第8天时有显著提高(P<0.05),AKP活性在第4、17天时有极显著提高(P<0.01),ACP活性在第12天时7.52%组有极显著提高(P<0.01),第17天时各浓度组均有显著或极显著提高(P<0.05或P<0.01)。研究表明,海带和条斑紫菜凝集素提取液均能提高剌参体壁中CAT、LSZ、AKP、ACP的活性,显著增强剌参非特异性免疫水平,将其作为刺参免疫增强剂具有广阔的应用前景。
The effects of two lectin solutions extracted from kelp Laminaria japonica and laver Porphyra yezoensis Veda on activities of immunoenzymes were studied in body walls of juvenile sea cucumber Apostichopus japonicus with body weight of（ 3. 50 ± 1. 95） g. The juveniles were fed basic diet（ as control group） supplemented with kelp lectin extracted solution at a rate 0. 64%,1. 28% and 2. 56%（ mass percent） and fed the basic diet supplemented with laver extracted solution at a rate 1. 88%,3. 76% and 7. 52%. The activities of catalase（ CAT）,lysozyme（ LSZ）,alkaline phosphatase（ AKP） and acid phosphatase（ ACP） were determined in the body walls on the 4th,8th,12 th and 17 th day after the sea cucumber juveniles were fed the diets supplemented with the kelp extracted solution,and on the 4th,8th,13 th and 17 th day after the juveniles were fed the diets supplemented with laver extracted solution. The results showed that the activities of CAT and LSZ were found to be significantly or very significantly increased in 8,12,and 17 d after the juveniles were fed the diets containing kelp extracted lectin solution group（ P〈0. 05 or P〈0. 01）. The AKP activity was shown to be significantly increased in the juveniles in 12 d in1. 28% kelp extracted lectin solution group（ P〈0. 01）,and ACP activity in 8,and 17 d in 0. 64% kelp extracted lectin solution group（ P〈0. 05）. The CAT activity was significantly or very significantly increased 8,13,and 17 d after the juveniles were fed supplemented with laver extracted lectin solution group（ P〈0. 05 or P〈0. 01）,LSZ activity significantly enhanced 8 d after the juveniles were fed supplemented with laver extracted lectin solution（ P〈0. 05）,and AKP activity very significantly increased 4,and 17 d in 7. 52% laver extracted lectin solution group（ P〈0. 01）. The activity of ACP was very significantly increased on the 12 th d in 7. 52% laver extracted lectin solution group（ P〈0. 01）,very significantly improved on the 17 th d in
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2014年第6期607-612,共6页
Journal of Dalian Ocean University
基金
辽宁省教育厅科研项目(2008T021)
国家自然科学基金资助项目(31071612)
关键词
刺参
海带
条斑紫菜
凝集素
免疫酶
Apostichopus japonicus
Laminaria japonica Aresch
Porphyra yezoensis Veda
lectin
immunoenzymes
