摘要
为了快速、准确地检测食品中多种病原菌的含量,设计了一种能够快速、定量、实时检测出食品中多种微生物含量的荧光生物传感器系统。采用LED灯作为激发光源,测量不同荧光量子点的微生物在不同发射波长的荧光强度,根据建立的定量检测模型,求得被测微生物含量;以STC89C52单片机为核心,包括键盘输入电路、光路模块控制电路、光电转换与放大电路、A/D转换电路、LCD显示及报警电路等;能定量检测出多种微生物含量,实现含量的LCD实时显示和超限报警。实验表明:系统操作简单,对福氏志贺氏菌检测误差在与荧光分光度计比较误差在5%以内,准确度高。
In order to rapidly, accurately and timely detect the pathogenic bacteria in food, a fluorescence biosensor was designed based on fluorescence quantum dot mark technology, photoelectric conversion principle, principle of C51 and integrated amplifier, etc. Using LED light as the excitation light, the detector measures the fluorescence intensity of different fluorescent quantum dots in different emission wavelengths, and the microbial content was calculated according to the quantitative detection model. Based on the MCU, the detecting system consists of keyboard input circuit, light path module controlling circuit, photovoltaic conversion and amplifying circuit, A/D conversion circuit, LCD displaying and alarming circuit, etc. The instrument is able to detect multiple types of microbes simultaneously, display the contents of microbes on LCD in real-time, and warn the operator with an alarm when the content is out of limit. The error is less than 5% in AFS compared with measurement error of Fu＇s Shigella. The accuracy of the system is high totally.
出处
《中国农机化学报》
2015年第1期285-288,293,共5页
Journal of Chinese Agricultural Mechanization
基金
杨凌现代农业国际研究院培育项目(A213021005)
关键词
病原菌含量
实时检测
荧光生物传感器
光电转换
多指标
pathogenic bacteria detection
detect timely
fluorescence spectrophotometer
photoelectric conversion
multi-index
