摘要
筛选能够抑制引起腹泻致病菌黏附的益生菌,并且对其适应肠道环境:耐酸、耐胆盐和黏附性能进行评价。从中国西部地区传统发酵食品以及人类粪便样品中分离的14株益生菌作为筛选菌株。对这14株益生菌进行抑制致病菌黏附的筛选,最终筛选出7株使致病菌黏附数量降低到1.5×104CFU/m L的益生菌进行耐受肠道环境能力评价。实验结果显示这7株益生菌在p H2的条件下处理90min后存活的益生菌依然能够达到107CFU/m L左右,在0.3%的胆盐环境中处理2h后菌数降低到原来的百分之一,单层细胞上黏附的益生菌数J5、G15和F0533能够达到106CFU/m L,显示出了较强的黏附能力。经过综合分析筛选出F0533、IN4125、G15、J5和M7益生菌,经16S r DNA基因技术鉴定分别为Lactobacillus rhamnosus、Lactobacillus rhamnosus、Lactobacillus paracasei、Lactobacillus casei和Lactobacillus paracasei。
In this study,fourteen selected Lactobacillus isolated from human intestinal and ferment milk were assessed the ability to inhibit the infection of enteropathogens. The Lactobacillus strains were screened on the basis of probiotic characteristics（i.e.,resistance to low p H and bile salts,adhesion to the human gastrointestinal tract）. By an in vitro system simulating gastric transit,it indicated that the three probiotic strains had the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells. It was demonstrated that the number of 7 probiotics strains could reach about 107CFU/m L after treatment of 90 min in p H2 and the number of bacteria strains decreased to one percent of the original after treatment of 0.3% of the bile salt environment 2h.Adhesion of probiotics on number of J5,G15 and F0533 could reach 106CFU/m L,which showed strong adhesion ability. Among the selected strains,five selected Lactobacillus showed a high probiotic potential and could be used in health-promoting food products. After analyzing the sequence of the 16 S r DNA regions of these strains,five potential probiotic F0533,IN4125,G15,J5 and M7 were Lactobacillus rhamnosus,Lactobacillus rhamnosus,Lactobacillus paracasei,Lactobacillus casei and Lactobacillus paracasei.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第2期227-230,242,共5页
Science and Technology of Food Industry
基金
国家自然科学基金资助项目(30871816/C120108)
关键词
益生菌
沙门氏菌
抑制粘附
耐酸耐胆盐
probiotics
Salmonella
inhibition of adhesion
resistance to low p H and bile salts
