摘要
目的研究柚皮素对肺腺癌A549细胞的增殖抑制及诱导凋亡作用。方法应用四甲基偶氮唑盐比色法(MTT法)观察柚皮素对A549细胞增殖的抑制作用;Hoechst 33342染色后于倒置荧光显微镜下观察柚皮素对A549细胞形态的影响;通过Annexin V-FITC/PI双染后流式细胞术检测柚皮素对A549细胞诱导凋亡的作用。结果柚皮素可抑制A549细胞的增殖,且呈现时效、量效依赖关系;Hoechst 33342染色倒置荧光显微镜下发现不同浓度柚皮素作用细胞48h后出现细胞核破裂、浓染,呈现明显的凋亡形态学特征;200μM、400μM柚皮素作用细胞48h后A549细胞凋亡率分别为(12.15±2.14)%和(25.26±3.37)%。结论柚皮素可能通过诱导细胞凋亡从而发挥其抑制A549细胞增殖的作用。
Objective To investigate induction of apoptosis and proliferation inhibition of naringenin on lung adenocar-cinoma A549 cells. Methods Used MTT assay to measure the inhibiting effect of naringenin on A549 cells proliferation. Ob-served the effect of naringenin on A549 cell morphology by Hoechst 33342 fluorescent staining on inverted fluorescence micro-scope. Detected induction of apoptosis of naringenin on A549 cells by flow cytometry after Annexin V-FITC/PI staining. Re-sults MTT results showed that the naringenin significantly suppressed the proliferation of A549 cells in time and concentration-dependent. Typical apoptotic morphological changes also appeared after different concentration naringenin treatment for 48h .Flow cytometry results demonstrated that the apoptosis rates of A549 cells were （12.15±2.14）%and （25.26±3.37）%after naringenin （200 and 400μmol/L） treatment for 48h,respectively. Conclusion Naringenin could inhibit A549 cells growth and prolifera-tion and induced apoptosis.
出处
《中国现代医药杂志》
2014年第10期19-21,共3页
Modern Medicine Journal of China
