摘要
[目的]研究冷热护理刺激对人宫颈癌 H eLa细胞克隆形成能力的影响及机制,以期探索冷热护理的新作用机制,促进临床护理水平的提高。[方法]HeLa细胞按常规用DMEM 高糖培养基培养；按6孔板每孔100个~300个细胞的浓度接种并在不同培养温度情况下进行细胞克隆试验；提取在不同温度环境下生长的同批次 HeLa细胞的总 RNA,在逆转录反应后获得 cDNA,以此为模板,实施荧光定量分析不同温度环境下 pan QKI mRNA的表达水平。[结果]较高的环境温度能增强 HeLa细胞的克隆形成能力,抑制 pan QKI mRNA表达；但较低的环境温度则抑制了该细胞的克隆形成,同时,pan QKI mRNA表达水平显著增高。[结论]温度干预能对人宫颈癌 HeLa细胞的克隆能力产生影响,pan QKI 在不同温度环境中的表达变化可能是导致 HeLa细胞克隆形成能力差别的重要原因,温度干预的护理效用和机制值得深入探究。
Objective：To probe into the influence of hot and cold care stimulus on forming ability of human cervi-cal cancer HeLa cells clone and its mechanism,so as to explore the new action mechanism of hot and cold care promote the improvement of clinical nursing level.Methods：HeLa cells were cultured by routine use of DMEM high glucose medium.According the concentration of 100~300 cells per hole,they were inoculated in 6 hole plate,cell cloning experiments were carried out in different culture temperature circumstances;the total RNA of the same batch of HeLa cells was extracted which grew in different temperatures,the cDNA obtained in the re-verse transcription reaction was taken as a template,the fluorescent quantitative analysis was implemented for expression level of pan QKI mRNA under different temperature environment.Results：The high ambient tem-perature can enhance the colony formation ability of HeLa cells and inhibit the pan QKI mRNA expression;but the lower ambient temperature inhibited the cell clone formation,at the same time,the expression level of mRNA was significantly increased in pan QKI.Conclusion：Temperature intervention can affect cloning ability of human cervical cancer HeLa cells.The changes of pan QKI expression in different temperature environ-ments may be an important reason of differences of HeLa cell clone forming ability.The efficacy and mechanism of temperature intervention are worth for in depth inquiry.
出处
《护理研究（上旬版）》
2015年第1期18-21,共4页
Chinese Nursing Researsh
基金
国家自然科学基金资助项目
编号:31271239
国家重点基础研究发展"973"计划资助项目
编号:2009CB941601
关键词
冷热护理
宫颈癌
H
eLa细胞
细胞克隆
护理干预
hot and cold care
cervical cancer
HeLa cell
cell clone
nursing intervention
