摘要
基于电化学阻抗技术构建了一种检测p53抑癌基因的电化学传感器。首先利用自组装作用将末端带巯基的探针p53抑癌基因（p53-DNA）固定于金电极表面,根据传感器结合前后电子转移阻抗值的变化,对目标p53-DNA进行了测定。以p H=7.4的5 mmol/L K3[Fe（CN）6]-5 mmol/L K4[Fe（CN）6]平衡电对溶液作为检测液,检测目标p53-DNA的线性浓度范围为1.0×10^-8~1.0×10^-6mol/L,其检出限为3.0×10^-9mol/L（S/N=3）。对5.0×10^-8mol/L的目标p53-DNA进行11次平行测定,其RSD为3.4%。该传感器制作简单、灵敏高、选择性好,且无需标记,易于操作。
A novel DNA sensor is designed for the determination of p53 tumor suppressor gene based on electrochemical impedance technology. First a thiol-end probe p53-DNA is immobilized onto the surface of gold electrode through the self-assembly technique, The electron transfer resistance of the DNA sensor is changed after the hybridization of complementary sequence p53-DNA, p53 tumor suppressor gene is quantitatively detected by the change in electron transfer resistance,in 5 mmoL/L K3 [ Fe（CN）6 ] -5 mmol/L K4 [ Fe（ CN）6 ] （ pH =7.4） solution, under the optimized conditions, the electrochemical impedance response is linear with the logarithm of the p53-DNA concentration from 1.0 × 10^-8 1.0 ×10^-6 mol/L with a detection limit of 3.0 × 10^-9 mo]/L （S/N = 3）. 11 times parallel determination is carried out on target p53-DNA of 5. 0 × 10^-8 tool/L, relation standard deviation（RSD） is 3.4 %. The sensor has advantages of easy fabrication, high sensitivity,good selectivity, and label free, easy to operate.
出处
《传感器与微系统》
CSCD
2015年第1期60-62,65,共4页
Transducer and Microsystem Technologies
基金
陕西省自然科学基础研究计划资助项目(2010JM2020)
关键词
电化学阻抗技术
传感器
P53抑癌基因
electrochemical impedance technology
sensor
p53 tumor suppressor gene
