摘要
目的：建立生物检材血液中农药的快速溶剂萃取（accelerated solvent extraction，ASE）法。方法通过优化ASE萃取条件，考察萃取温度、时间和萃取剂对回收率的影响，提取血液中的农药进行气相色谱-质谱联用（GC/MS）法定性定量分析。结果血液中8种农药的平均回收率在70.6%～92.4%，变异系数小于5.0%，8种农药在0.5～5.0μg/mL的浓度范围内线性良好。结论该方法具有操作简便快捷、回收率高、重现性好等特点，可用于农药的提取检验。
Objective To develop the accelerated solvent extraction （ASE ） for determ ining pesticides pre-sent in blood sam ples. Methods Pesticides were extracted by ASE with optimized param eters to study recovery rate affected by extraction tem perature, time and agent. GC/MS was used to perform quantita-tive analysis.Results The recovery rates of eight pesticides were 70.6%-92.4%. The coefficient of variation was less than 5.0%. Agood linear relationship was obtained at the concentration range of 0.5-5.0μg/m L . Conclusion The m ethod was fast and sim ple with high recovery rate and good repeatability. It can be applied to analyze pesticides present in the blood specimen.
出处
《法医学杂志》
CAS
CSCD
2014年第6期463-465,共3页
Journal of Forensic Medicine
关键词
法医毒理学
气相色谱-质谱法
快速溶剂萃取
农药
血液
forensic toxicology
gas chromatography-mass spectrometry
accelerated solvent extraction
pesticides
blood
