摘要
目的建立沉香药材HPLC特征指纹图谱分析方法,为其科学鉴定和质量控制提供依据。方法 Dionex-Acclaim 120C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.5%甲酸水溶液梯度洗脱;体积流量0.4 m L/min;检测波长254 nm;柱温26℃。利用"中药色谱指纹图谱相似度评价系统2004A版"和SPSS软件分别对其进行相似度评价和聚类分析。结果建立了沉香药材HPLC指纹图谱共用模式,确定24个共有峰,并根据对照品指认9个共有峰。10批沉香药材相似度分析结果与聚类分析结果基本一致。结论首次建立了沉香药材HPLC指纹图谱分析方法。该分析方法简便、快速,可较全面地反映沉香药材中化学成分的信息,为沉香药材的鉴定和质量评价提供了科学依据。
Objective To establish an HPLC method for the fingerprint analysis of Aquilariae Resinatum Lignum（ARL）, so as to provide evidence for the identification and quality control of ARL. Methods The analysis was carried out on a Dionex-Acclaim 120 C18 column（250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile and water-acetic acid（99.5：0.5） with the flow rate of 0.4 m L/min at 254 nm and the separation was performed at 26 ℃. The similarity was analyzed with ＂Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004A＂ and the cluster analysis was performed by SPSS software. Results The HPLC characteristic fingerprint of ARL has been established. A total of 24 common peaks were characterized, and nine of them were identified by comparing their retention time with reference subslances. The values of similarity evaluation mostly agreed with the result of cluster analysis. Conclusion It is the first time to establish the HPLC fingerprint of ARL. The method is simple and quick, and reflects the information of chemical composition of ARL comprehensively, which provides the scientific basis for the identification and quality evaluation of ARL.
出处
《中草药》
CAS
CSCD
北大核心
2014年第23期3456-3461,共6页
Chinese Traditional and Herbal Drugs
基金
国家科技支撑计划课题(2013BAI11B04)
海南省重大科技项目(ZDZX2013013)
公益性行业(农业)科研专项(201303117)
关键词
沉香
HPLC指纹图谱
相似度分析
聚类分析法
超声波法
Aquilariae Resinatum Lignum
HPLC fingerprint
similarity analysis
cluster analysis
ultrasonic method
