摘要
以实验室筛选的一株高产纤维素酶的黑曲霉(Aspergillus niger)LG11作为出发菌株,进行响应面分析。在前期单因素试验基础上,设计Plackett-Burman试验,确定影响产酶的显著因素:发酵时间、装液量和转速;通过最陡爬坡试验逼近最大响应值区域,最后通过Box-Behnken响应面设计试验进行回归分析,得出最佳发酵条件为:发酵时间78 h,500m L三角瓶装液64 m L,转速190 r/min,优化后羧甲基纤维素酶(Carboxymethyl cellulase,CMCase)活力由57.1 U/m L提高到74.6U/ml,提高30.65%,试验结果为纤维素酶工业化生产提供了理论依据。
Response surface methodology was adopted to optimize cellulase fermentation conditions by Aspergillus niger LG11, which is a screened strain by our Laboratory. Based on these results of previous single factor experiments, the important factors including fermentation time, volume of the medium and speed influencing the Carboxymethyl cellulase （CMCase） activity were identified by Plackett-Burman factorial design. The steepest ascent experiment was adopted to approach the optimal response value region. Finally, the optimal fermentation conditions were obtained by Box-Behnken design and regression analysis as follows： the optimal medium conditions were 64 mL medium in 500 mL flask, 190 r/min for 78 h. Under these conditions, CMCase activity reached 74.6 U/mL, which increased by 30.65% than that before the optimization （57.1 U/mL）.
出处
《山东食品发酵》
2014年第4期35-40,共6页
Shandong Food Ferment
关键词
纤维素酶
黑曲霉
响应面法
Cellulase
Aspergillus niger
Response surface methodology
