摘要
主要介绍了单克隆抗体药物工业生产中宿主细胞选择、表达载体构建、转染方法、筛选技术、细胞培养工艺技术方法以及最后选定细胞株的标准等,结合单抗药物CHO细胞株开发和培养工艺的经验,对当前我国单抗CHO细胞株开发技术策略进行了探讨。
Several strategies for industrial production of monoclonal antibody drug were introduced in this paper, including host cell selection, construction of expression vector, transfection method, screening techniques, cell culture technology as well as the standard method of final cell lines selection. On the basis of the progress of monoclonal antibody drug CHO cell line development and cell culture process, current strategy for the development of CHO cell lines in monoclonal antibody technology were also discussed.
出处
《生物技术进展》
2014年第6期394-399,共6页
Current Biotechnology
基金
上海市科学技术委员会"科技型中小企业技术创新资金"项目(1402H195100)资助
关键词
单克隆抗体
CHO细胞
细胞株开发
培养工艺
monoclonal antibody
CHO cell
cell line development
cell culture process
