摘要
荧光PCR技术的高敏感性导致检测结果易出现假阳性和假阴性。阳性样本和阳性对照污染是导致假阳性的原因;RNA酶降解核酸是导致假阴性的重要原因。避免出现假阳性和假阴性检测结果需从实验室设置和管理、操作人员和试验器材管控入手。
The high sensitivity of fluorescence PCR is easily lead to false positive and false negative. False positive is due to pollution from positive samples and the positive control. Rnase degradation is an important factor of false negative. False positive and false negative is avoided by improved laboratory setup and management, operating personnel and test equipment control.
出处
《广东畜牧兽医科技》
2014年第6期50-51,共2页
Guangdong Journal of Animal and Veterinary Science
关键词
荧光PCR
假阳性
假阴性
Fluorescence PCR
false positive
false negative
