摘要
通过体外细胞培养对"灵巴菌质"(巴豆发酵品)的石油醚部位和碱性醇提物的抗肿瘤活性进行初步筛选。对"灵巴菌质"以石油醚、二氯甲烷、水饱和正丁醇和甲醇四个极性梯度依次进行抽提,得到石油醚部位(PEE)等4个提取部位;甲醇提取物再采用酸溶碱沉-溶剂萃取等方法处理,得到其碱性醇提物(AME)。通过快速、灵敏的MTT比色法考察石油醚部位和碱性醇提物对SMMC-7721、MGC-803、A549、Hep G-2肿瘤细胞增殖的影响。PEE、AME与巴豆苷(Cro)一样,对A549细胞增殖均有显著抑制作用,且呈浓度依赖性;而二者对MGC-803细胞活力没有明显影响。在一定浓度范围内,AME仅对SMMC-7721细胞增殖有抑制作用;PEE和AME对Hep G-2细胞增殖却有促进作用。"灵巴菌质"的石油醚部位和碱性醇提物对SMMC-7721、MGC-803、A549、Hep G-2等4种瘤株增殖的影响是有一定选择性的,二者仅对A549细胞生长均呈现出明显的抑制作用。
Croton fruits（Euphorbiaceae） has been applied for the treatment of some types of cancer in traditional Chinese medicine for a long time.Lingba Junzhi is a fermented product of croton fruits by Ganoderma lucidum （Curtis：Fr.） P.Karst.The paper was fixed to screen the effective fractions about resisting tumor in Lingba Junzhi in vitro.Soxhlet extraction was used to extract Lingba Junzhi with the gradient solvents,and four extract fractions were obtained,petroleum ether extracts（PEE）,dichloromethane extracts,normal butanol extracts and methanol extracts.Then methanol extracts were dealt with the alkali-solution and acid-isolation and solvent extraction,to prepare alkaline methanol extracts （AME）.Screening anti-tumor effects of PEE and AME to SMMC-7721,MGC-803,A549,HepG-2 tumor cells were investigated by means of quick and sensitive MTT colorimetry.Compraring with crotonoside （Cro）,PEE and AME inhibited the proliferation of A549 cells significantly in a dose-dependent manner,and had nearly no influence on MGC-803 cells.Within a certain concentration range,AME played a strong inhibited effect only to the SMMC-7721 proliferation,while PEE and AME worked for the promotion of the HepG-2 proliferation.All these mentioned above suggest that the effects on proliferative activity of different parts of Lingba Junzhi is quite different.But PEE and AME all have the obvious anti-tumor effects on A549 cell proliferation.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2014年第11期1216-1221,共6页
Journal of Food Science and Biotechnology
基金
江苏高校优势学科建设工程资助项目(ysxk-2010)
江苏省中医药局科技项目(LZ09018)
关键词
巴豆发酵品
抗肿瘤
抗肿瘤活性部位
细胞培养
fermented products of Croton
anti-tumor
chemical components
cell culture
