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anti-CD3单抗包被培养技术培养DC-CIK细胞的研究

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摘要 目的比较采用anti-CD3 单抗包被培养技术与传统方法培养的DC-CIK 细胞在体外扩增、活细胞数量、细胞纯度、免疫表型及体外杀伤能力的差异,来选择更优的DC-CIK 细胞培养方法.方法:抽取健康志愿者肝素抗凝外周血,采用anti-CD3 单抗包被培养技术与传统方法培养DC-CIK 细胞,检测细胞总数、活细胞数量、细胞纯度、免疫表型及体外杀伤能力.结果:采用包被技术培养的DC-CIK 细胞数量第7、10、13 天分别是常规方法的2.15、2.14、2.10 倍(P 〈0.05); 活细胞百分率包被组为(99.1±0.2)%,传统组为(98.5±0.3)%;细胞纯度包被组为(98.8±0.1)%,传统组为(98.1±0.2)%;免疫表型和体外杀伤能力包被组均优于传统组,有统计学差异.结论: anti-CD3 单抗包被培养技术相比传统DC-CIK 细胞培养技术更优.
出处 《中医学报》 CAS 2014年第B12期415-415,共1页 Acta Chinese Medicine
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