摘要
目的了解2013年山东省四地市A群链球菌(GAS)耐药特征及其耐药表型和基因型,为临床治疗GAS感染提供依据。方法从济南、青岛、淄博、潍坊四地市的猩红热、无症状GAS携带者咽拭子中分离获得72株GAS,采用K-B纸片扩散法检测青霉素、红霉素、克林霉素、左氧氟沙星、四环素、氯霉素、万古霉素、头孢噻肟8种抗生素的耐药情况;提取DNA,PCR扩增erm A、erm B、mef A、tet M、tet O共5种耐药基因,毛细管电泳检测基因阳性率;利用D-抑菌圈试验确定耐药表型。结果 72株GAS中,青霉素、左氧氟沙星、氯霉素、万古霉素、头孢噻肟敏感率均为100%,红霉素、克林霉素、四环素耐药率为100%、100%、94.44%。共检出erm B、tet M两种耐药基因,其阳性率均为100%,未检出erm A、mef A、tet O。仅检出一种耐药表型:固有型耐药表型(c MLS)检出率100%,未发现诱导型耐药表型(i MLS)和主动外排型耐药表型(M)。结论 2013年山东省GAS菌株中,耐药模式以红霉素-克林霉素-四环素共同耐药为主,耐药表型以c MLS为主,耐药基因型以erm B、tet M共同携带为主。
Objective To investigate the characteristics of antibiotic resistance,resistance phenotypes and genotypes on group A streptococcus （GAS）isolated from children in 4 cities of Shandong Province in 2013 and to provide reference for clinical treatment of GAS infection.Methods A total of 72 strains of GAS were isolated from throat swabs of chil-dren with scarlet fever or asymptomatic carriers.The resistance of GAS strains on 8 antibiotics,including penicillin,e-rythromycin,clindamycin,levofloxacin,tetracycline,chloramphenicol,vancomycin and cefotaxime,were measured with K-B disk-diffusion method.DNA was extracted from GAS strains,resistance genes （ermA,erm B,mefA,tetM, tetO）were amplified with PCR method,then positive rates of genes were assessed with capillary electrophoresis.The＆nbsp;resistance phenotypes were detected with text.Results Among the 72 strains of GAS,all isolates were susceptible to penicillin,levofloxacin,chloramphenicol,vancomycin and cefotaxime.The resistance rates of erythromycin,clinda-mycin and tetracycline were 100%,100%and 94.44%,respectively.Two resistance genes,erm Band tetM,were de-tected （both 100%）,while ermA,mefA and tetO were not.Only cMLS phenotype was found in all isolates （100%）, while iMLS and M phenotype were not （0%）.Conclusion The resistance pattern of GAS in 4 cities of Shandong Province in 2013 was multiple antibiotic resistance of erythromycin-clindamycin-tetracycline.The resistance phenotype was cMLS,and the genotypes were mainly co-carried erm Band tetM.
出处
《山东大学学报（医学版）》
CAS
北大核心
2015年第1期77-80,共4页
Journal of Shandong University:Health Sciences
基金
国家科技重大专项(2012ZX10004215)
关键词
链球菌
酿脓
耐药
药敏试验
耐药基因
儿童
山东省
Streptococcus pyogenes Drug resistance Bacterial susceptibility testing Resistance genes Children Shandong Province
