摘要
目的建立测定经血宁胶囊中伞房决明苷含量的高效液相色谱法。方法采用Capcell Pak C18柱（250 mm×4.6 mm,5μm）,以磷酸盐混合溶液（称取磷酸氢二钠1.8 g,磷酸二氢钾2.8 g,和庚烷磺酸钠1.0 g,加水稀释至1 L,混匀,用磷酸调节p H至6）-甲醇（75∶25）为流动相,检测波长为335 nm,柱温30℃,流速为1.0 mL/min。结果伞房决明苷进样量在0.001 991～0.995 5μg范围内与峰面积线性系良好（r=0.999 6）,平均回收率为99.08%,RSD=1.88%（n=9）。结论该法专属性强、重现性好,适用于经血宁胶囊中伞房决明苷的定量分析。
Objective To establish a HPLC method for the determination of Cassia corymbosa glycoside content in Jingxuening Capules.Methods The analytical column was a Capcellpak-C18（ 250 mm × 4. 6 mm, 5 μm）. The mobile phase consisted of methanol-phosphate mixed solution（ dissolving disodium hydrogen phosphate 1. 8 g, potassium dihydrogen phosphate 2. 8 g and heptane sulfonic acid sodium1. 0 g in water and adding water to 1L,adjusting to p H 6. 0 by phosphoric acid）（ 25 ∶ 75）. The detection wavelength was 335 nm. The column temperature was 30 ℃ and the flow rate was 1. 0 mL / min. Results The linear range of Cassia corymbosa glycoside was in the range of 0. 001 991- 0. 995 5 μg, r = 0. 999 6, the average recovery rate was 99. 08% with RSD 1. 88%（ n = 9）. Conclusion This method has strong specificity and good repeatability, and is suitable for the content determination of Cassia corymbosa glycoside in Jingxuening Capules.
出处
《中国药业》
CAS
2015年第1期51-52,共2页
China Pharmaceuticals
