摘要
目的:研究p43蛋白对干扰素诱导蛋白10(IP10)的调控。方法:用实时定量PCR方法检测p43蛋白作用于HMEC-1细胞后IP10基因水平的变化,并找到p43蛋白作用的最佳浓度范围及最佳作用时间;同时用ELISA方法检测p43蛋白是否促进IP10蛋白的分泌,并检测p43蛋白对可能引起IP10上调的相关因子γ干扰素(IFNγ)、白细胞介素1β(IL1β)及肿瘤坏死因子α(TNFα)的调控作用,试图找到p43蛋白诱导IP10表达上调的原因。结果及结论:p43蛋白能显著上调IP10的表达,并具有一定的量效关系,在p43浓度为70μg/m L、作用时间为6 h时达到峰值;p43蛋白能够诱导IFNγ的分泌,p43蛋白诱导IP10的表达可能是通过上调IFNγ的分泌,并经由JAK-STAT途径实现的。
Objective： To study the regulation of p43 protein on the interferon inducible protein 10（IP10）. Methods： With the method of real-time quantitative PCR to detect the IP10 gene level changes of p43 pro？tein on HMEC-1 cells, and find the best role of p43 protein concentration range, and the best reaction time; and detect the IP10 protein secretion measured by ELISA. p43 protein may lead to increase of the relevant fac？tors IFNγ, IL1β and TNFα, so we detect whether the p43 protein could increse those factors. Trying to find the regulation of p43 protein on IP10. Results ＆amp; Conclusion： p43 protein can significantly increase the expression of IP10, and with a certain dose-effect relationship. The optimal concentration of p43 was 70 μg/ml, and when role 6 h the IP10 have a significant secretion. While p43 protein can induce the secretion of IFNγ protein. IP10-induced expression may be through increased IFNγ protein secretion, and achieve through the way of JAK-STAT.
出处
《生物技术通讯》
CAS
2014年第6期779-782,共4页
Letters in Biotechnology
