摘要
目的观察壮尔颗粒(Zhuang'er Granules,ZG)对细胞免疫抑制小鼠免疫器官指数及T淋巴细胞亚群的影响。方法用环孢菌素A(Cs A)建立小鼠模型,并在检测前第7天滴鼻肺炎链球菌(SP)。灌胃给药17 d后,测定小鼠免疫器官指数,并采用流式细胞仪(FACS)检测T淋巴细胞亚群的变化。结果胸腺指数与脾脏指数同空白对照组相比,模型组均有明显下降(P<0.05),各给药组与模型组比较均有明显提高(P<0.05),并随着给药量的增加而升高(P<0.05)。脾脏中CD3+,CD4+,CD8+与空白对照组相比,模型组CD3+明显下降,CD4+有下降趋势,CD8+有上升趋势;各给药组CD3+,CD4+百分率随着给药剂量增加而升高,而CD8+的百分率随着给药剂量增加而降低。血中CD3+,CD4+,CD8+与空白对照组相比,模型组CD3+有下降趋势,CD4+明显下降(P<0.05),CD8+明显升高(P<0.05),各给药组CD3+和CD4+的百分率随着给药剂量的增加而升高(P<0.05),而CD8+的百分率随着给药剂量的增加而降低。结论壮尔颗粒有提高小鼠免疫功能的作用,提高CD3+和CD4+的同时,降低CD8+,以通过T细胞的平衡来调节小鼠自身的免疫功能。
Objective To observe the effect of Zhuang＇er Granules on the immune organ index and T- lymphocytes subsets in the cellular immunosupressed mice. Methods The mouse model was established by cyclosporin A（Cs A） and given Streptococcus pneumoniae（SP） by nasal drops on 7 d before detection. The mouse immune organ index was determined after 17 d intragastric gavage and the changes of T- lymphocyte subsets were detected by the flow cytometry（FACS）. Results Compared with the blank control group,the thymus index and spleen index in the model group were obviously decreased（P〈0. 05）; compared with the model group,which in each dose group were obviously increased（P〈0. 05）,and obviously increased with dose increase（P〈0. 05）; compared with the blank control group,for CD3^＋,CD4^＋,CD8^＋in the spleen,CD3^＋in the model group was declined obviously（P〈0. 05）,CD4^＋showed the decline trend and CD8^＋model group had the rising trend; the percentage of CD3^＋,CD4^＋in each dose group was increased with dose increase,while the percentage of CD8^＋was declined with dose increase; compared with the blank control group,for blood CD3^＋,CD4^＋,CD8^＋,CD3^＋in the model group showed the decline trend,CD4^＋was obviously decreased（P〈0. 05） and CD8^＋was obviously increased（P〈0. 05）,the percentage of CD3^＋,CD4^＋in each medication group was increased with dose increase（P〈0. 05）,while the percentage of CD8^＋in each medication group was decreased with dose increase. Conclusion Zhuang＇er Granules has the effect for improving the mice immune function,increases CD3^＋and CD4^＋,simultaneously decreases CD8^＋,which could regulate the mice immune function via T- lymphocytes balance.
出处
《中国药业》
CAS
2014年第24期35-37,共3页
China Pharmaceuticals
基金
辽宁省沈阳市科技局重点项目
项目编号:F11-264-1-73
