摘要
采用体积分数95%乙醇进行加热回流提取后,根据极性由小到大分别用正己烷、二氯甲烷、乙酸乙酯、正丁醇(有机溶剂∶水=1∶1)萃取.利用乙酸乙酯层提取物,经HPLC进一步分离纯化后得到较多的纯馏分.纯化后的馏分经LC-MS进行结构鉴定-定性分析.结果表明:根据质谱信息定性分析馏分1为杨梅素.该方法灵敏、准确、简便.
Using 95% ethanol heated and refluxed for extraction;respectively using hexane,dichloromethane,ethyl acetate,n-butanol according to the polarity from small to large（organic solvent∶water =1∶1）.For the sake of more pure fractions,we adopt HPLC to further purification with the extract of ethyl acetate layer.Purified fractions structural identification-qualitative analysis by LC-MS.Based on the mass information carry on qualitative analysis, we found that fraction 1 is myricetin.The method is sensitive,accurate,and convenient.
出处
《河南科技学院学报（自然科学版）》
2014年第5期39-42,共4页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
