摘要
目的对胶乳增强免疫比浊法定量测定血清心型脂肪酸结合蛋白(H-FABP)的方法进行临床实验评价。方法分析胶乳增强免疫比浊法测定H-FABP的精密度、线性范围、前带反应、回收率和干扰因素,并考察其与酶联免疫吸附法的相关性。结果胶乳增强免疫比浊法测定血清H-FABP批内精密度的高值与低值变异系数分别为0.54%、1.56%;批间精密度的高值与低值变异系数分别为0.58%、1.8%,回收率为96.2%~99.3%。线性测定良好,可检测范围为2.5 ng/m L^160 ng/m L,标准品曲线回归方程为Y=1.02X+0.31,标准品检测相关性R2≥0.995。跟国外同类试剂相比较,曲线回归方程为Y=0.97X+0.13,检测相关性R2=0.998。实验组血清H-FABP检测结果为(78.3±10.4)ng/m L,与对照组相比差异有统计学意义。结论胶乳增强免疫比浊法测定血清H-FABP快速、准确、可靠,适用于临床急性心肌梗死的早期诊断。
Objective To do clinical experimental evaluation on serum heart -type fatty acid binding protein （H -FABP） immunoas- say method for latex - enhanced turbidimetric immunoassay. Methods H - FABP immunoassay precision latex - enhanced turbidimetry, linear range, response, recoveriy rate and confounding factors were analyzed, and the relationship between ELISA and it was examed. Resuits The high and low values CV% of the latex - enhanced immunoturbidimetric assay serum H - FABP assay precision were 0.54% and 1.56% ; inter - assay precision CV% of high and low values were O. 58% and 1.8%, and the recovery was 96.2% - 99.3%. Linear measurement was good, and could be detected in the range of 2.5 ng/mL - 160 ng/mL. The standard curve regression equation was Y = 1. 02X ＋ 0. 31, and standard detection correlation was R2 ≥0.995. Compared with similar foreign agents, curve regression equation was Y = 0.97X ＋0.13, with a correIation R2 =0. 998. The serum H -FABP test result was （78.3 ±10.4）ng/mL, with statistically significant differences with the control group. Conclusion Immunoturbidimetric assay of serum H - FABP latex - enhancement is fast, accurate, relia- ble and suitable for clinical evaluation of acute myocardial infarction.
出处
《黑龙江医学》
2014年第11期1250-1252,共3页
Heilongjiang Medical Journal
关键词
胶乳增强免疫比浊法
心型脂肪酸结合蛋白
急性心肌梗死
方法学评价
Latex - enhanced turbidimetric immunoassay
Heart - type fatty acid binding protein
AMI
Methodological evaluation
