摘要
目的以SPF级APP/PS1双转基因小鼠(22周)为阿尔茨海默病(AD)动物模型,皮下注射胰岛素样生长因子-1(IGF-1),观察其对AD模型小鼠脑内淀粉样蛋白(Aβ1-40)表达的影响。方法将APP/PS1双转基因小鼠及隐性基因小鼠分为4组,设立隐性基因组、隐性基因治疗组、转基因组及转基因治疗组。隐性基因治疗组、转基因治疗组分别皮下注射IGF-1[50μg/(kg·d)],共8周。随后,各组行免疫组化方法观察小鼠脑内Aβ1-40的表达。结果转基因组及转基因治疗组可见神经元缺失。同转基因组比较,无论是皮质区还是海马区,转基因治疗组脑内Aβ1-40的表达均明显减少(P<0.05)。结论 IGF-1有降低APP/PS1双转基因小鼠脑内Aβ1-40表达的作用,具有阻断和延缓AD模型脑内老年斑形成的作用,尤其是对皮质区的Aβ1-40表达的阻断更为明显。
Objective To investigate the effects of subcutaneous injection of insulin like growth factor-1（ IGF-1 ） on the expression of amyloid protein（Aβ1-40） in the APP/PS1 double transgenic mice. Methods APP/PS1 double trans- genic mice and recessive mice were respectively divided into 4 groups, the establishment of recessive genome group, reces- sive gene therapy group, genome group and transgenic therapy group. Suhcutaneous injection of IGF-1 [ 50 μg/（ kg · d） ] were given to both the recessive gene therapy group and transgenic therapy group. After 8 weeks, the expression of the Aβ1-40 in the cortical area and hippocampal area of mouse was detected by immunohistochemical methods. Results The genome group and transgenic therapy group also exhibited irregular arrangement of neuron units. The expression of Aβ1-40 in the cortical and hippocampal area in the transgenic therapy group was significantly lower than that in genome group （P〈 0. 05）. Conclusions Through decreasing the expression of Aβ1-40 in the cortical and hippocampal area, IGF-1 might inhibit the damage induced by Aβ1-40 in the AD mice model.
出处
《实用老年医学》
CAS
2014年第11期906-909,共4页
Practical Geriatrics
基金
黑龙江省留学归国科学基金项目(LC2009C13)
