摘要
以水解度为主要指标,筛选了动物蛋白酶、海参水解酶、木瓜蛋白酶、水产蛋白酶、中性蛋白酶、复合蛋白酶、风味蛋白酶、胰蛋白酶和碱性蛋白酶对仿刺参的酶解效果,考察了酶解温度、加酶量、水解时间、p H等单因素条件对水解度的影响,在此基础上通过响应面分析获得仿刺参的最佳酶解工艺,并对最佳工艺条件下酶解液高、中、低剂量对小鼠改善睡眠的功能进行了研究。结果表明:水产蛋白酶对仿刺参蛋白的水解效果优于其他受试蛋白酶,且响应面结果显示其加酶量对水解度具有极显著影响(p<0.01);响应面法优化得到刺参酶解的最适条件为:p H7、温度49℃、加酶量8%(酶/底物百分比)、时间4h,其水解度达到28.0%;仿刺参酶解液高、中、低剂量组对小鼠没有明显的直接催眠作用,但中剂量组能显著增加阈下剂量戊巴比妥钠诱导的小鼠睡眠发生率(p<0.05)。
The degree of hydrolysis was taken as the main index to screen animal protease,sea cucumber hydrolase,papain,aquatic protease,neutrase,protamex,flavourzyme,trypsin and alcalase for hydrolysis of sea cucumber Apostichopus joponicus. Based on the effect of single factors enzymolysis temperature,dosage of enzyme,hydrolyzation time and pH value on the degree of hydrolysis,the analysis of response surface method were carried out to optimize the hydrolysis process,and the sleep improvement function of the hydrolysate of Apostichopus joponicus at low,middle and high dosage obtained under optimal conditions were carried out. The results showed that the degree of aquatic protease hydrolysis was obviously higher than other enzymes tested in this paper. The effect of enzyme dosage on the degree of hydrolysis（DH） was significant（p〈0.01 ） by the analyse of SPSS. By the RSM transactional analysis,the optimum enzymolysis conditions were optimized as followspH7,enzymolysis temperature 49℃ ,the ratio of enzyme to substrate 8% and time 4h,respectively. The DH was up to 28.0% under the optimum condition. The aquatic protease hydrolysate of sea cucumber Apostichopus joponicus had no effect to sleep-induced directly in rat,but in middle dosage it could obviously prolong the rats＇ sleep time which induced by pentobarbital sodium at under-threshold dosage（p〈0.05）.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第24期198-202,207,共6页
Science and Technology of Food Industry
基金
山东省科技发展计划项目(2012GHY11512)
关键词
仿刺参
酶解
水解度
响应面
改善睡眠
Apostichopus joponicus
enzymolysis
degree of hydrolysis
response surface method
sleepimprovement
