摘要
为了探究长时间重金属镉胁迫对芦苇抗氧化能力的影响,采用实验室桶栽模拟试验,对长时间序列下芦苇叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)活性对不同浓度镉胁迫的响应关系进行研究,建立镉胁迫下SOD、POD活性变化动力学方程,并计算其参数。结果表明:一定浓度的镉污染处理有利于提高SOD、POD活性;SOD、POD活性随镉浓度的增加而增加,在各生理阶段均与镉浓度呈正相关。模型参数计算表明:镉浓度与芦苇叶片SOD活性内禀增长率正相关,与环境容纳量、米氏常数、拐点时刻均负相关;在Cd3、Cd4、Cd5水平下,镉浓度与芦苇叶片POD活性内禀增长率、环境容纳量、米氏常数、拐点时刻均正相关。当镉浓度为2mg·kg-1时,POD活性内禀增长率最小,环境容纳量与米氏常数均最大,拐点时刻为11.27,综合SOD、POD拐点时刻结果,镉处理促进SOD、POD活性的极限浓度为2mg·kg-1,在此浓度下,芦苇仍能对镉胁迫做出有效的反应,对芦苇生长量影响不大。
In order to explore the effects of cadmium stressing on the antioxidant capacity of reeds, barrel simulation experiments were done to research the responsing relationship between cadmium stress and activities of supemxide dismutase （SOD/peroxidase （POD） in reeds＂ leaves for long terms, the dynamics equation of SOD/POD activities was established, and the parameters were calculated. The results showed that the SOD/POD activities are improved as treated by certain concentration of cadmium, the SOD/POD activities were increasing while the cadmium concentration increased, positive correlation was showed at all physiological stages of reeds. The model parameter calculation showed that cadmium concentration was positively correlated with the intrinsic rates of SOD activity in reeds＂ leaves, while negatively correlated with the environmental capacity, michaelis constant and inflection point. The relationship was positive between cadmium concentration and the four indices under C3, C4 and C5 condition. Under C2 condition, the intrinsic rates of POD activity reacheed minimum, environmental capacity and michaelis constant reaches maximum, inflection point was 11.27. Comprehensive SOD, POD inflection point results indicated the limit cadmium concentration was 2mg·kg^-1 which could improve the SOD and POD activities. At that concentration, the reeds could make effective response to cadmium stress and the effect on its own growth was small.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2014年第3期326-330,共5页
Journal of Shenyang Agricultural University
基金
国家自然科学基金青年基金项目(31100517)
辽宁省优秀人才支持计划项目(LJQ2011070)
辽宁省教育厅科学技术研究项目(2009A628)
