摘要
目的观察温针灸治疗兔膝关节退变模型前后血清基质金属蛋白酶1、13(MMP-1,MMP-13)及软骨形态的变化,探讨温针灸治疗膝骨性关节炎的作用机理。方法 30只雄性兔随机分为模型组、温针灸组和西药组,每组10只,采用右后肢伸直位固定制作兔膝关节退变模型。采用双抗体夹心酶联免疫吸附法(ELISA)检测造模前、治疗前和治疗后兔血清中MMP-1,MMP-13水平,参考彭太平法观察膝关节组织形态学变化。结果治疗2周后,大体和光镜观察模型组膝关节软骨变薄,出现明显的关节软骨损伤,温针灸组可明显改善关节软骨组织学结构。各组治疗前较造模前血清MMP-1、MMP-13的浓度均显著升高(P<0.05);治疗两周后,与模型组比较,温针灸组MMP-1、MMP-13水平降低(P<0.05),但与西药组比较,差异无统计学意义(P>0.05)。结论温针灸能治疗KOA的软骨损伤,可能与降低MMP-1和MMP-13表达有关。
Objective To investigate the influence of needle warming moxibustion on matrix metalloprotenases and histomorphology of the rabbit experimental knee osteoarthritis model, and to explore its relative mecha- nism. Methods 30 male rabbits were randomly divided into model, needle warming moxibustion and western medicine group, each group of 10 cases. The concentrations of MMP - 1, MMP - 13 in serum were detected by ELISA after two weeks treatment. Results The cartilage became thinning and appeared a significant articular injury. Needle warming moxibustion group can improve the histological structure of articular cartilage. MMP - 1 and MMP - 13 before treatment were increased significantly compared with normale （ P 〈 0.05 ）. Compared with model group,both MMP- 1 and MMP- 13 levels in warm acupuncture- moxibustion group were reduced considerablly （ P 〈 0.05 ）. No significant differences were found between western medication group and model group, western medication group and needle warming moxibustion group. Conclusion MMP - 1, MMP - 13 participated in the occurrence of KOA and needle warming moxibustion can treat KOA ar- ticular injury, which may related with the expression of MMP - 1 , MMP - 13.
出处
《宁夏医科大学学报》
2014年第5期497-499,504,F0003,共5页
Journal of Ningxia Medical University
基金
国家自然科学基金(81260545)
