摘要
对2012—2013年浙江省凡纳滨对虾(Litopenaeus vannamei)主要养殖区的红体病虾进行病原鉴定及其分子特征与耐药性研究,并与2011年的结果进行比较分析.结果表明:通过Vitek、16SrRNA序列分析与病毒特异性聚合酶链反应,2012—2013年在红体病虾中同时检测出副溶血弧菌(Vibrio parahaemolyticus)与传染性皮下和造血组织坏死病毒(infectious hypodermal and haematopoietic necrosis virus,IHHNV),而2011年在红体病虾中仅检测出副溶血弧菌,未检测出IHHNV;3年均未检测出桃拉综合征病毒(Taura syndrome virus,TSV)和白斑综合征病毒(white spot syndrome virus,WSSV).基于dnaE-gyrB-recA-dtdSpntA-pyrC-tnaA的多位点序列分型结果表明,副溶血弧菌分离株具有较高水平的分子多样性:2011—2013年各年的分离株均分属多个序列型(sequence type,ST),来自位于进化树不同亚枝、亲缘关系较远的不同克隆;2012—2013年的分离株经历了较高水平的分子变异,其中ST918与ST919为首次报道的副溶血弧菌新ST;2011年分离株与2012—2013年分离株的等位基因和序列型特征具有较大差异,ST414与ST114分别代表2011年与2012—2013年红体病的优势ST.2011—2013年的副溶血弧菌分离株均属于大流行群,并具有相同的毒力基因谱(tlh+tdh-trh-T3SS1+T3SS2-),tdh与trh的缺失并未影响细菌的致病力.分离株对抗菌药物尤其是氨基糖苷类的耐药性呈现加重趋势.说明本次凡纳滨对虾红体病可能是多种致病因子共同作用的结果,亟须建立红体病病原监测体系,并探明细菌性病原与病毒性病原混合感染对致病性的影响及其互作机制,以提出综合防控方案.
Summary Pacific white shrimp ( Litopenaeus vannamei) is one of the most important commercially cultured aquaculture species around the world . Zhejiang Province represents as one of the main cultured areas in China . In recent years , concurrent with the rapid expanding and intensifying of aquaculture , infectious diseases in Pacific white shrimp L . vannamei have been steadily increasing . Red‐body disease is one of the most common and severe diseases of Pacific white shrimp . From 2011 to 2013 , recurrent outbreaks of Pacific white shrimp red‐body disease occurred in large‐scale breeding farms within the main cultured area of Zhejiang Province , which caused severe economic losses to the shrimp culture industry . Affected shrimps showed typical signs of red bodies , irregular black spots , listless swimming on water surface and reduced feed activities , accompanying with mass mortalities .This study was conducted to investigate the bacterial and viral pathogens from 2012 - 2013 outbreaks and to illuminate their molecular characteristics and antimicrobial sensitivities , which were compared with those from 2011 outbreak . Using Vitek biochemical test , 16S rRNA sequence analysis and virus specific polymerase chain reaction , V ibrio parahaemolyticus and infectious hypodermal and haematopoietic necrosis virus ( IHHNV ) were simultaneously identified from the diseased Pacific white shrimps of 2012 - 2013 outbreaks , but except V . parahaemolyticus , IHHNV was not detected from the diseased ones of 2011 outbreak ; meanwhile , Taura syndrome virus ( TSV) and white spot syndrome virus ( WSSV) were not detected from the diseased Pacific white shrimps of 2011 - 2013 outbreaks . Although V . parahaemolyticus was positive from all outbreaks during three years , these isolates exhibited remarkable genetic diversity by multilocus sequence typing ( MLST ) based on the concatenated genes dnaE‐gyrB‐recA‐dtdS‐pntA‐pyrC‐tnaA . The isolates from the same year belonged to multiple sequence types ( STs) , origina
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2014年第6期697-708,共12页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
农业部“浙江省水生动物疫病监控中心”重大建设项目
浙江省海洋与渔业局“水产养殖病害测报”专项(334006-2010-0006)
关键词
凡纳滨对虾
副溶血弧菌
传染性皮下和造血组织坏死病毒
分子特征
耐药性
Litopenaeus vannamei
Vibrio parahaemolyticus
infectious hypodermal and haematopoietic necrosis virus
molecular characteristics
antimicrobial sensitivity
