摘要
目的分析DKK1在大鼠骨髓间充质干细胞成脂、成骨分化早期的表达,探讨其调节机制。方法 Real-time PCR检测骨髓间充质干细胞在成脂、成骨诱导培养早期DKK1基因表达的水平;检测激素处理骨髓间充质干细胞DKK1基因的表达水平。结果成脂组与正常对照组,在成脂诱导6、12、24、48时DKK1基因表达水平较对照组增高,差异有统计学意义,P<0.01。成骨组与正常对照组,0.5、6两个时间点DKK1基因表达量较对照组明显降低,差异有统计学意义,P<0.05。骨髓间充质干细胞激素处理3、6、12、24时检测,结果显示激素作用下,骨髓间充质干细胞DKK1表达明显增加,差异有统计学意义,P<0.05。结论 DKK1在骨髓间充质干细胞成脂、成骨分化早期可能起重要的调节作用,激素可能通过调节DKK1的表达而发挥调节成脂、成骨分化的作用。
Objective To analyzed the DKK1 expression in early osteogenic and adipogenic differentiation of BMSCs in rats and to explore its regulating mechanism.Methods DKK1 gene expression in early adipogenic and osteogenic differentiation was detected using real-time PCR.And the DKK1 expression was also detected after hormone treatment.Results The expression level of DKK1 gene after 6, 12, 24, and 48h adipogenic induction was higher than that in control group and the difference was significant （P＆lt;0.01）.The expression level of DKK1 gene after 0.5 and 6h osteogenic induction was obviously lower than that in control group and the difference was significant （P＆lt;0.05）.The expression level of DKK1 gene of BMSCs after 3, 6, 12, and 24 h hormone treatment was higher than in control group （P＆lt;0.05）.Conclusion DKK1 plays an important role in early osteogenic and adipogenic differentiation by BMSCs.The hormone affects osteogenic and adipogenic differentiation by regulating the expression of DKK1.
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2014年第11期1290-1297,共8页
Chinese Journal of Osteoporosis
关键词
DKK1
骨髓间充质干细胞
糖皮质激素
成骨分化
成脂分化
WNT信号
DKK1
Bone marrow mesenchymal stem cells
Glucocorticoid
Osteogenic differentiation
Adipogenic differentiation
Wnt signaling
