摘要
目的:探讨牙本质屏障法用于牙齿充填材料体外细胞毒性试验测定的特点。方法:低速锯片切新鲜拔除的磨牙,获取紧邻牙髓的颊侧牙本质片,选择通透值相近的牙本质片作为牙本质屏障,制作细胞毒性体外测试装置,用此装置测定氧化锌丁香油水门汀、复合树脂、玻璃离子水门汀、氢氧化钙水门汀、磷酸锌水门汀及阴性对照材料和阳性对照材料的细胞毒性,同时用传统的浸提液法测定这5种材料的细胞毒性。结果:牙本质屏障法测定的5种材料的细胞相对活性(%)分别为89.1±3.6、81.8±3.7、96.4±2.8、96.2±1.8、74.5±2.8;浸提液法测定的5种材料的浸提原液细胞相对活性(%)分别为35.6±5.2、72.9±4.3、91.5±1.3、90.2±2.6、59.3±2.6。结论:牙本质屏障可以显著减少充填材料对细胞的毒性作用,牙本质屏障法更能反映充填材料的体内细胞毒性。
AIM： To compare cell reaction to five common dental filling materials tested with an indirect contact method equipped with dentin barrier and a conventional extraction culture method. METHODS： Dentin discs with 0.5mm in thickness were cut from the buccal inner dentin of freshly extracted molars and were determined for hydraulic permeability. The discs with similar permeability were used as barrier to prepare device for cytotoxicity test in vitro. Cytotoxicity of composite resin, ZOE, GIC, Ca（OH） 2 and ZPC cement, was tested using both this device and conventional extraction culture method with MTT assay. RESULTS ： Relative cell viability （ RCV, % ） obtained with dentin barrier for the five filling materials were ： 81.8 ± 3.7, 89.1 ± 3.6, 96.4 ± 2.8, 96.2 ± 1.8 and 74.5 ± 2.8 respectively. The RCV（ % ） obtained with extraction culture method were 72.9 ± 4.3, 35.6 ± 5.2, 91.5 ± 1.3, 90.2 ± 2.6 and 59.3 ± 2.6 respectively. CONCLUSION： Dentin barrier reduced the toxicity of filling material to cells, and the results from dentin barrier method are more reliable in reflecting the in vivo toxicity of filling materials.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2014年第11期625-629,共5页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金项目(81171002)
