摘要
为摸清大鳞鲃在碱性水域推广的可行性,对大鳞鲃(Barbus capito)、草鱼(Ctenopharyngodon idllus)、镜鲤(Songpu mirror carp)、鲫鱼(Carassius auratus)和白鲢(Hypophthalmichthys molitrix)幼鱼进行96 h急性NaHCO3碱度毒性试验,测定了水体中NaHCO3碱度对5种鱼的致死浓度,以及对鳃、肾组织的影响。结果显示,大鳞鲃、草鱼、镜鲤、鲫鱼和白鲢5种幼鱼NaHCO3碱度96 h半致死浓度LC50分别为123.325 mmol/L,91.825 mmol/L,114.262 mmol/L,134.045 mmol/L,86.246 mmol/L,其耐受能力大小顺序依次为:鲫鱼,大鳞鲃,镜鲤,草鱼,白鲢。观察了NaHCO3碱度对鳃和肾脏的病理组织切片,在高碱胁迫下,鳃小片缩短,细胞间隙增大,鳃小片上皮细胞出现不同程度的水肿,甚至脱落;肾组织中肾小管出现不同程度的萎缩,肾小管管腔变大。
In order to verify the promoted feasibility of Barbus capito in alkaline waters,experiments were conducted to explore the half lethal concentrateion （ LC50 ） of NaHCO3 in juveniles of 5 species through 96 h a-cute alkalinity toxicity test with fresh water test for comparison,and its the impact on gill,kidney tissues was ob-cerved.According to the results of the acute alkalinity experiment,the LC50 of alkalinity in Barbus capito,Ctenopha-ryngodon idllus, Songpu mirror carp, Carassius auratus, and Hypophthalmichthys molitrixwere 123.325 mmol/L, 91.825 mmol/L,114.262 mmol/L,134.045 mmol/L,86.246 mmol/L,respectively.Therefore,the sequence of alkali-tolerance was：Carassius auratus〉Barbus capito〉Songpu mirror carp〉Ctenopharyngodon idllus〉Hypoph-thalmichthys molitrix.Furthermore,the gill and kidney structures of 5 juveniles were observed under NaHCO3 by using the technology of histological photomicrography.The results showed that gill lamellae narrowed,inter-cellular space increased and edema appeared in the epithelial cells in different degrees,the cells or even fell off;atrophy appeared in renal tubules in different degrees.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2014年第5期1115-1121,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家"十二五"科技支撑计划项目(2012BAD25B09)
中央级公益性科研院所基本科研业务费专项(201007
201108)
哈尔滨市科技攻关计划项目(2012AA6CN037)
关键词
幼鱼
碱度
半致死浓度
鳃
肾
juveniles alkalinity LC50 gill kidney
