摘要
为建立莴苣花叶病毒的现场快速检测方法,采用胶体金标记莴苣花叶病毒的兔多克隆抗体,在硝酸纤维素膜上喷涂检测T线和质控C线,制作胶体金免疫层析试纸条。该试纸条检测莴苣花叶病毒提纯病毒的灵敏度为1μg·mL-1,检测昆诺阿藜病汁液可稀释1 000倍(重量/体积)。试纸条可特异性检测到莴苣花叶病毒的3个分离物,与马铃薯X等8种病毒无交叉反应。田间采集的莴苣样品试纸条和酶联检测的结果一致。该试纸条适用于疑似莴苣花叶病毒感染的莴苣叶片的快速诊断,并在10min内获得检测结果。
In order to establish a rapidly diagnostic method for Lettuce mosaic virus（LMV）in the field,collioidal gold immunochromatographic strip（ICS）was developed by coupling colloidal golds with polyclonal rabbit antibody against LMV.Test line and control line on nitrocellulose membrane were spread by Biojet Quanti 3 000 dispenser on XYZ dispensing platform.The sensitivity of immunostrip was 1μg·mL^-1for purified LMV.Extract from LMV infected Chenopodium quinoaleaf diluted 1 000 times could be used to detect（W/V）.The immunostrip was specific to LMV and detected three isolated viruses.There was no cross reaction with other eight viruses.Immunostrip test result was confirmed by ELISA for lettuce leaves collecting from field.The immunostrip was suitable for rapid diagnosis of LMV in suspicious lettuce leaf samples within 10 minutes.
出处
《黑龙江农业科学》
2014年第11期74-77,共4页
Heilongjiang Agricultural Sciences
基金
质检公益性行业科研专项资助项目(201310071)
