摘要
通过对海洋13个样品元基因组数据的BLAsT搜索,筛选到了16s rRNA基因序列1600条,18s RNA基因序列61条。分类结果显示,细菌在海岸、公开海域深层海水和表层海水3种海洋环境类型中都占优势,其相对百分比分别为98%、59%和91%。相比于海岸和公开海域表层海水,公开海域深层海水中古生菌和Deltaproteobacteria所占的相对含量较高,各31%和27%。海岸检测到的古生菌主要为Euryarcheata,公开海域深层海水检测到的古生菌主要为Crenarchaaeota(93%为与氨氧化相关的MGI纲)。结果表明,氨氧化相关古生菌在深海生态系统中的作用可能较大。
Based on BLAST searching of metagenomic data of 13 marine samples,1 600 pieces of 16S rRNA gene sequences and 61 pieces of 18S rRNA gene sequences were screened.Classification results showed that the bacteria was dominant in coast,open sea waters,deep sea water and surface sea waters,the relative percentage was 98,59 and 91 respectively.As compared with coast and surface of open sea waters,the contents of Archaea and Deltaproteobacteia were relatively high,they accounted for 31% and 27% respectively.Archaea determined and tested in coast mainly was Euryarchaea,while Archaea determined and tested in open deep sea waters mainly was Crenarchaeota,and 93% of which was correlated to ammonia oxidizing related class of MGI.The results of this study showed that ammonia oxidation related Archaea may play a larger role in eco-system in deep open seas.
出处
《微生物学杂志》
CAS
CSCD
2014年第5期8-12,共5页
Journal of Microbiology
基金
国家自然科学基金面上项目(31170114)