摘要
目的通过逆转录聚合酶链反应(RT-PCR)方法探讨姜黄素对脂多糖(LPS)刺激成骨细胞后骨保护因子(OPG)和破骨细胞核因子κB受体活化因子配基(RANKL)m RNA表达的影响。方法 1μg/m L LPS作用成骨细胞6、12、18、24、48 h,并用10μmol/L姜黄素预处理成骨细胞60 min后加入1μg/m L LPS继续作用6、12、18、24、48 h,RT-PCR检测成骨细胞OPG和RANKLm RNA的表达。结果当以1μg/m L LPS作用于成骨细胞,随着作用时间的增加成骨细胞表达RANKL m RNA的能力逐渐增强,到24 h达到最高峰,到48 h基本恢复至初始水平;用10μmol/L姜黄素预处理成骨细胞,然后再用1μg/m L LPS处理成骨细胞48h,发现成骨细胞表达RANKL m RNA的能力明显减弱,随着作用时间的增加成骨细胞表达RANKL m RNA的能力变化不明显。当以1μg/m L LPS作用于成骨细胞,成骨细胞表达OPG m RNA的能力无明显变化;用10μmol/L姜黄素预处理成骨细胞,然后再用1μg/m L LPS处理成骨细胞48 h,发现成骨细胞表达OPG m RNA的能力也无明显变化。结论姜黄素可以减少LPS刺激后成骨细胞RANKL m RNA的表达,对OPG m RNA表达无影响,进而使得RANKL/OPG的比值减小,抑制骨吸收。
Objective To investigate the effect of curcumin on the expression of osteoprotegerin （OPG） and receptor activator of nuclear factor-κB ligand （RANKL） mRNA stimulated by lipopolysaccharide （LPS） in osteoblasts by reverse transcription-polymerase chain reaction （RT-PCR）.Methods Osteoblasts with/without 10 μmol/L curcumin precondition were treated with 1 μg/mL LPS for 6,12,18,24,48 h.RT-PCR was employed to detect the mRNA expression of OPG and RANKL.Results A time dependent increase in RANKL mRNA expression was observed in 24 h when cells were treated with 1 μg/mL LPS.The level of RANKL mRNA began to decrease after 24 h.The expression of RANKL mRNA in osteoblasts was similar as that in untreated controls at 48 h.Before exposure to LPS,osteoblasts were pretreated with 10 μ mol/L curcumin,the expression of RANKL mRNA decreased significantly and exhibited no time dependent relationship,the level of RANKL mRNA slightly changed.The level of OPG mRNA slightly changed when osteoblnsts either treated with 1 μg/mL LPS or 10 μmol/L curcumin preconditioned until 48 h.Conclusion Curcumin suppressed bone resorption by downregulate the expression of RANKL mRNA induced by LPS in osteoblasts and had no effect on that of OPG mRNA.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2014年第11期982-985,共4页
Journal of China Medical University
基金
辽宁省科技计划项目(2013225090)
