摘要
目的 探讨辛伐他汀对大鼠视网膜缺血再灌注损伤中的保护机制.方法 实验研究.选取健康成年SD雄性大鼠165只,采用随机数字表法随机分为对照组、模型组和药物组,每组55只,每组分4、8、16、24、48 h,总共5个时间点,各时间点11只.模型组、药物组以夹闭右侧颈总动脉的方法造模,对照组暴露右颈总动脉不夹闭.采用免疫组织化学方法检测Bcl-2和Bax的蛋白表达水平,采用TUNEL法检测视网膜各层的细胞凋亡情况,用Real Time RT-PCR方法检测Bcl-2和Bax mRNA的表达水平.对各组大鼠视网膜相应时间点的Bcl-2和Bax的蛋白及mRNA的表达及细胞凋亡情况进行比较采用单因素方差分析和LSD-t检验.结果 模型组Bcl-2于4h开始下降,在24h达到最低值,分别为(0.192 ±0.011)、(0.192 ±0.015)、(0.189±0.015)、(0.183 ±0.012)、(0.187 ±0.010).药物组各时间点均高于模型组,分别为(0.208±0.011)、(0.220±0.011)、(0.221 ±0.014)、(0.228 ±0.007)、(0.206 ±0.015).各时间点比较,差异有统计学意义(F4.8,16.24,48=8.079,9.005,9.904,35.563,8.810,P<0.05).模型组Bax从4h开始上升,24 h达到高峰,分别为(0.255±0.010)、(0.261 ±0.033)、(0.276±0.025)、(0.324±0.037)、(0.234 ±0.018).药物组低于模型组,分别为(0.222 ±0.012)、(0.219 ±0.017)、(0.223 ±0.008)、(0.232±0.021)、(0.214 ±0.008).各时间点比较,差异均有统计学意义(F4,8,16,24,48=16.601,13.525,10.303,25.849,13.805,P<0.05).模型组细胞凋亡指数从4h开始上升,24 h达到高峰,分别为(11.771 ±0.722)、(13.705 ±0.512)、(15.533 ±0.370)、(21.210±1.221)、(17.660±0.414).药物组低于模型组,分别为(9.061±0.289)、(11.717 ±0.565)、(13.506±0.541)、(16.586±0.488)、(14.428±0.559).各时间点比较,差异均有统计学意义(F4,8,16,24,48=87.096,232.245,575.564,389.205,771.658,P<0.05).Bcl-2mRNA在模型组�
Objective To explore the protective mechanism of simvastatin on retina ischemiareperfusion injury in a rat model.Methods It was a experiment study.One hundred and sixty-five adult male SD rats were randomly divided into three groups using digital table method,normal control group (CON,55 rats),ischemia-reperfusion model group(MOD,55 rats) and the medicine of simvastatin group (SIM,55 rats).Each group was divided into five points in time of 4 hours,8 hours,16 hours,24 hours and 48 hours,and there were 11 rats in each point.The right cephalic artery of each rat was clipped in model group and simvastatin group,but it was exposed in control group.Expression of Bcl-2 and Bax protein were determined by the immunohistochemical method,the number of cell apoptosis in retina were examined by the TUNEL method and express of Bcl-2 and Bax mRNA were measured by the real-time PCR method.The expression of proteins and mRNAs of Bcl-2 and Bax and also apoptosis of the rat retinas in each group at corresponding time point are compared using single factor analysis of variance and LSD-t test.Results Expression of Bcl-2 protein in model group began to decline at 4 h,reached the lowest at 24 h,with the data of(0.192 ±0.011),(0.192 ±0.015),(0.189 ±0.015),(0.183 ±0.012)and(0.187 ±0.010).Expression of Bcl-2 protein in simvastatin group were higher than model group at each time point,with the information of (0.208 ± 0.011),(0.220 ± 0.011),(0.221 ± 0.014),(0.228 ± 0.007) and (0.206 ± 0.015).The numbers were statistically significant at corresponding time point in each group (F4,8,16,24,48 =8.079,9.005,9.904,35.563,8.810,P 〈 0.05).Expression of Bax protein in model group began to increased at 4 h,reached the highest at 24 h,with the data of(0.255 ± 0.010),(0.261 ± 0.033),(0.276 ± 0.025),(0.324 ± 0.037) and (0.234 ± 0.018).Expression of Bax protein in simvastatin group were lower than model group at each time point,with the information of (0.222 ±
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2014年第11期826-832,共7页
Chinese Journal of Ophthalmology