期刊文献+

辛伐他汀对大鼠视网膜缺血再灌注中Bcl-2和Bax表达及细胞凋亡的影响 被引量:13

Effect of simvastatin on retinal Bcl-2/Bax expression and cell apoptosis in rats with ischemia-reperfusion injury
原文传递
导出
摘要 目的 探讨辛伐他汀对大鼠视网膜缺血再灌注损伤中的保护机制.方法 实验研究.选取健康成年SD雄性大鼠165只,采用随机数字表法随机分为对照组、模型组和药物组,每组55只,每组分4、8、16、24、48 h,总共5个时间点,各时间点11只.模型组、药物组以夹闭右侧颈总动脉的方法造模,对照组暴露右颈总动脉不夹闭.采用免疫组织化学方法检测Bcl-2和Bax的蛋白表达水平,采用TUNEL法检测视网膜各层的细胞凋亡情况,用Real Time RT-PCR方法检测Bcl-2和Bax mRNA的表达水平.对各组大鼠视网膜相应时间点的Bcl-2和Bax的蛋白及mRNA的表达及细胞凋亡情况进行比较采用单因素方差分析和LSD-t检验.结果 模型组Bcl-2于4h开始下降,在24h达到最低值,分别为(0.192 ±0.011)、(0.192 ±0.015)、(0.189±0.015)、(0.183 ±0.012)、(0.187 ±0.010).药物组各时间点均高于模型组,分别为(0.208±0.011)、(0.220±0.011)、(0.221 ±0.014)、(0.228 ±0.007)、(0.206 ±0.015).各时间点比较,差异有统计学意义(F4.8,16.24,48=8.079,9.005,9.904,35.563,8.810,P<0.05).模型组Bax从4h开始上升,24 h达到高峰,分别为(0.255±0.010)、(0.261 ±0.033)、(0.276±0.025)、(0.324±0.037)、(0.234 ±0.018).药物组低于模型组,分别为(0.222 ±0.012)、(0.219 ±0.017)、(0.223 ±0.008)、(0.232±0.021)、(0.214 ±0.008).各时间点比较,差异均有统计学意义(F4,8,16,24,48=16.601,13.525,10.303,25.849,13.805,P<0.05).模型组细胞凋亡指数从4h开始上升,24 h达到高峰,分别为(11.771 ±0.722)、(13.705 ±0.512)、(15.533 ±0.370)、(21.210±1.221)、(17.660±0.414).药物组低于模型组,分别为(9.061±0.289)、(11.717 ±0.565)、(13.506±0.541)、(16.586±0.488)、(14.428±0.559).各时间点比较,差异均有统计学意义(F4,8,16,24,48=87.096,232.245,575.564,389.205,771.658,P<0.05).Bcl-2mRNA在模型组� Objective To explore the protective mechanism of simvastatin on retina ischemiareperfusion injury in a rat model.Methods It was a experiment study.One hundred and sixty-five adult male SD rats were randomly divided into three groups using digital table method,normal control group (CON,55 rats),ischemia-reperfusion model group(MOD,55 rats) and the medicine of simvastatin group (SIM,55 rats).Each group was divided into five points in time of 4 hours,8 hours,16 hours,24 hours and 48 hours,and there were 11 rats in each point.The right cephalic artery of each rat was clipped in model group and simvastatin group,but it was exposed in control group.Expression of Bcl-2 and Bax protein were determined by the immunohistochemical method,the number of cell apoptosis in retina were examined by the TUNEL method and express of Bcl-2 and Bax mRNA were measured by the real-time PCR method.The expression of proteins and mRNAs of Bcl-2 and Bax and also apoptosis of the rat retinas in each group at corresponding time point are compared using single factor analysis of variance and LSD-t test.Results Expression of Bcl-2 protein in model group began to decline at 4 h,reached the lowest at 24 h,with the data of(0.192 ±0.011),(0.192 ±0.015),(0.189 ±0.015),(0.183 ±0.012)and(0.187 ±0.010).Expression of Bcl-2 protein in simvastatin group were higher than model group at each time point,with the information of (0.208 ± 0.011),(0.220 ± 0.011),(0.221 ± 0.014),(0.228 ± 0.007) and (0.206 ± 0.015).The numbers were statistically significant at corresponding time point in each group (F4,8,16,24,48 =8.079,9.005,9.904,35.563,8.810,P 〈 0.05).Expression of Bax protein in model group began to increased at 4 h,reached the highest at 24 h,with the data of(0.255 ± 0.010),(0.261 ± 0.033),(0.276 ± 0.025),(0.324 ± 0.037) and (0.234 ± 0.018).Expression of Bax protein in simvastatin group were lower than model group at each time point,with the information of (0.222 ±
作者 张玉 颜华
出处 《中华眼科杂志》 CAS CSCD 北大核心 2014年第11期826-832,共7页 Chinese Journal of Ophthalmology
关键词 再灌注损伤 视网膜疾病 斯伐他汀 原癌基因蛋白质C-BCL-2 BCL-2相关X蛋白质 细胞凋亡 Reperfusion injury Retinal diseases Simvastatin Proto-oncogene proteins c-bcl-2 bcl-2-Associated X protein Apoptosis
  • 相关文献

参考文献4

二级参考文献44

  • 1Hollenhorst RW.Ocular manifestations of insufficiency or thrombosis of the internal carotid artery.Am J Ophthalmol,1959,47:753-767. 被引量:1
  • 2Alizai AM,Trobe JD,Thompson BG,et al.Ocular ischemic syndrome after occlusion of both external carotid arteries.J Neuroophthalmo1,2005,25:268-272. 被引量:1
  • 3Kearns TP,Hollenhorst RW.Venous stasis retinopathy of occlusive disease of the carotid artery.Proc Staff Meet Mayo Clin,1963,38:304-312. 被引量:1
  • 4Hedges TR Jr.Ophthalmoscopic findings in internal carotid artery occlusion.Bull Johns Hopkins Hosp,1962,3:111:89-97. 被引量:1
  • 5Young LH,Appen RE.Ischemic oculopathy:a manifestation of carotid artery disease.Arch Neurol,1981,38:358-361. 被引量:1
  • 6Brown GC,Magargel LE.The ocular ischemic syndrome:clinical,fluorescein angiographic and carotid angiographic features.Int Ophthalmol,1988,11:239-251. 被引量:1
  • 7张惠蓉.颈内动脉供血不足的眼部表现[J].中华眼科杂志,1983,5:308-310. 被引量:1
  • 8Worrall M,Atebara N,Meredith T,et al.Bilateral ocular ischemic syndrome in Takayasu disease.Retina,2001,21:75-76. 被引量:1
  • 9Kiyosawa M,Baba T.Ophthalmological findings in patients with Takayasu disease.Int J Cardiol,1998,66 Suppl:S141-147. 被引量:1
  • 10Sharma S,Brown GC.Ocular ischemic syndrome//Ryan Sj.Retina.Vol 2.4th ed.Philadelphia:Elsevier,2006:1491-1502. 被引量:1

共引文献53

同被引文献105

引证文献13

二级引证文献42

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部